|
|||||
|
|
| 嗅鞘细胞与神经修复支架材料的相互作用 | |
| 引用本文： | 王永红,殷义霞,李世普,严琼娇,万志涛,韩颖超. 嗅鞘细胞与神经修复支架材料的相互作用[J]. 中南大学学报(医学版), 2009, 34(5): 382-387 |
| 作者姓名： | 王永红 殷义霞 李世普 严琼娇 万志涛 韩颖超 |
| 作者单位： | 武汉理工大学医院内科,武汉,430070;武汉理工大学生物材料与工程研究中心,武汉,430070;武汉理工大学生物材料与工程研究中心,武汉,430070 |
| 基金项目： | supported by the Key Project of Chinese National Programs for Fundamental Research and Development(2005CB623905) |
| 摘 要： | 目的：探讨一种提取高纯度和一定数量的嗅鞘细胞(OECs)的新方法，并研究其与神经支架修复材料PLGL的生物相容性。方法：从新生的 Wister 大鼠身上分离制备嗅鞘细胞并与PLGL共同培养。测量其接触角、吸附率、存活率等。结果：PDLLA的接触角（84.5°±1.5°）明显大于PLGL的接触角（52.6°±0.8°）。PLGL的吸附率（80%）明显高于PDLLA（57%）。PLGL的细胞存活率（88%）明显高于PDLLA（76%）。结论：PLGL比PDLLA具有更好的亲水性、存活率以及良好的细胞生长状况。 |
| 关 键 词： | 嗅鞘细胞 脊神经损伤 PLGL 神经修复材料 生物相容性 |
| 收稿时间： | 2009-02-19 |
Interaction of olfactory ensheathing cells with nerve repairing scaffolds |
|
| WANG Yonghong,YIN Yixia,LI Shipu,YAN Qiongjiao,WAN Zhitao,HAN Yingchao. Interaction of olfactory ensheathing cells with nerve repairing scaffolds[J]. Journal of Central South University. Medical sciences, 2009, 34(5): 382-387 | |
| Authors: | WANG Yonghong YIN Yixia LI Shipu YAN Qiongjiao WAN Zhitao HAN Yingchao |
| Affiliation: | 1.Department of Internal Medicine, Hospital of Wuhan University of Technology, Wuhan 430070; 2.Biomedical Materials and Engineering Research Center, Wuhan University of Technology, Wuhan 430070, China |
| Abstract: | Objective To investigate a new way to yield plenty of high purity olfactory ensheathing cells (OECs) and its biocompatibility with appropriate scaffolds.Methods OECs were prepared from neonatal Wister rats and co-cultured with poly [LA-co-(Glc-alt-Lys)] (PLGL). Its contact angle, adherent rate, and activity rate were tested.Results The contact angle of poly (D, L-lactic acid) (PDLLA)(84.5°±1.5°) was significantly higher than that of PLGL (52.6°±0.8°), the adherent rate of PLGL (80%) was significantly higher than that of the PDLLA (57%), and the activity rate of PLGL(88%) was much higher than that of the PDLLA (76%).Conclusion PLGL possesses better hydrophilicity and biocompatibility than PDLLA, and it can provide a better cell growth circumstance which is helpful for the effective treatment of nerve injury. |
| Keywords: | PLGL olfactory ensheathing cells (OECs) spinal cord injury (SCI) poly [LA-co-(Glc-alt-Lys)] (PLGL) nerve repairing scaffold biocompatibility |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中南大学学报(医学版)》浏览原始摘要信息 | |
| 点击此处可从《中南大学学报(医学版)》下载全文 | |