| 胶束液相色谱法测定血清中硝苯地平的含量 |
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| 引用本文： | 陈丽娟 张瑶纾. 胶束液相色谱法测定血清中硝苯地平的含量[J]. 中国药房, 2005, 16(19): 1482-1483 |
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| 作者姓名： | 陈丽娟 张瑶纾 |
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| 作者单位： | 天津市胸科医院药剂科 天津市300051(陈丽娟)，天津医科大学 天津市300070(张瑶纾) |
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| 摘 要： | 目的:建立以胶束液相色谱法测定人血清中硝苯地平含量的方法。方法:色谱柱为C18,流动相为甲醇-水(60∶40,内含25mmol/L的SDS),检测波长为248nm,灵敏度为0.01AUFS,流速为1.0ml/min,记录纸速为2.1mm/min。结果:硝苯地平检测浓度在4～40ng/ml范围内线性关系良好(r=l.0002),平均回收率为98.15%(RSD=1.49%)。结论:本方法操作简便、准确、易行、重现性好,可为硝苯地平的临床血药浓度监测提供一种新的方法。
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| 关 键 词： | 胶束液相色谱法 硝苯地平 血清 含量 |
| 文章编号： | 1001-0408（2005）19-1482-02 |
| 收稿时间： | 2005-04-05 |
| 修稿时间： | 2005-08-27 |
Content Determination of Serum Nifedipine by Micell Liquid Chromatography |
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| Chen LiJuan;Zhang YaoShu. Content Determination of Serum Nifedipine by Micell Liquid Chromatography[J]. China Pharmacy, 2005, 16(19): 1482-1483 |
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| Authors: | Chen LiJuan Zhang YaoShu |
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| Abstract: | OBJECTIVE:To establish micell liquid chromatography for the content determination of nifedipine in human serum.METHODS:The determination was performed on C 18 chromatographic column,the mobile phase consisted of methanol-water(60∶40,which containing20mmol/L SDS),the detection wavelength was248nm,the sensitivity was0.01AUFS,the flow rate was1.0ml/min and the chart speed was2.1mm/min.RESULTS:The linear range for nifedipine was4～40ng/ml(r=1.0002),the average recovery was98.15%(RSD=1.49%).CONCLUSION:The method is simple,accuˉrate,feasible,reproducible,and it can be used as a new method for the monitoring of clinic haemoconcentration of nifedipine. |
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| Keywords: | Micell liquid chromatography Nifedipine Serum Content |
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