数字流体微量喷射系统的设计与实现

针对生物及医药工程领域微系统等相关研究,并实现微喷射过程在线观测,从数字化微量喷射技术原理出发,将计算机视觉和智能控制技术与之融合,设计了数字流体微量喷射实验系统的结构和工作台运动控制与微量喷射控制软件平台;利用单因素法和机器视觉技术,通过数字流体喷点测试和微阵列制作实验,研究驱动电压对微喷射的影响及样品点尺度均一性,并对实验数据进行分析和总结。结果表明:样品点直径随驱动电压增大而增大,基本成线性关系,最小可达60μm左右;样品点像素的相对变动量基本上集中在1附近,完全可以满足生物微阵列样品点尺度及均一性要求;微阵列喷点基本圆整、排列规则,符合微阵列规整度要求;验证了系统的合理性与可行性。

Design and Implementation of the Digital Fluid Micro-Jetting System

In order to monitor the micro-jetting process online for the microsystem research in biomedical engineering related fields,we designed a new experimental digital micro-jetting system which combined the machine vision technology with the intelligent control technology.This paper introduced the structure design and the control platform design for worktable motion and micro-jetting of this system.Then using a single factor analysis method and machine vision technology,we made a microarray jetting experiment to study the effects of the driving voltages on micro-jetting and the sample scale uniformity to demonstrate whether this system is feasible and reasonable.The testing results showed that the diameter of the sample spots increased with the increase of the driving voltage and kept a linear relationship basically,and the diameters could be below to 60 μm,and the relative variations in pixels of the sample spots were distributed near 1,and that the spots in microarray were almost round and arranged regularly,which completely satisfy the requirements for the biological microarray sample spots of the uniformity,size,regularity;verified the rationality and feasibility of the system.