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| 肝癌细胞线粒体的纯化及双向凝胶电泳分析 | |
| 引用本文： | 李兴,邱宗荫,潘卫,田昆仑,邱峰. 肝癌细胞线粒体的纯化及双向凝胶电泳分析[J]. 重庆医科大学学报, 2005, 30(2): 163-165,177 |
| 作者姓名： | 李兴 邱宗荫 潘卫 田昆仑 邱峰 |
| 作者单位： | 1. 重庆医科大学医学检验系,重庆,400016 2. 贵阳医学院医学检验系,贵阳,550004 3. 第三军医大学野战外科研究所,重庆,400016 |
| 摘 要： | 目的:建立纯化肝癌细胞线粒体的方法,通过双向电泳及酶活力测定确认纯化效果.方法:使用蔗糖密度梯离心法分离纯化线粒体,双向凝胶电泳分析纯化过程中样品蛋白质图谱的变化.结果:通过标志酶活力的监测,梯度离心后线粒体纯提高近12倍,线粒体蛋白质在双向凝胶电泳中得到很好的显现.结论:使用蔗糖密度梯度心法可有效地分离纯化线粒体,为线粒体蛋白质组后续研究奠定了基础. |
| 关 键 词： | 线粒体 梯度离心 双向凝胶电泳 |
| 文章编号： | 0253-3626(2005)02-0163-03 |
IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells |
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| LI Xing,et al. IsoIation and 2-DE analysis of mitochondria of cultured hepatoma cells[J]. Journal of Chongqing Medical University, 2005, 30(2): 163-165,177 | |
| Authors: | LI Xing et al |
| Abstract: | Objective:To make the isolation and 2-DE analysis of mitochondria,in order to prepare the following proteomic study on mitochondria of hepatoma-cell. Methods:Mitochondria was separated from cell homogenate by means of density gradient centrifugation,and 2-DE was conducted to examine the protein profile of mitochondrial preparations.Results:The increase in purity of mitochondria was found to be 12 times by density gradient centrifugation.Mitochondrial proteins were displayed well in the 2-DE pattern after purification.Conclusion:The isolation procedure is practicable,which provides a basis for the following proteomic study on mitochondria. |
| Keywords: | Mitochondria Density gradient centrifugation Two-dimensional gel electrophoresis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |