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A rapid immunoperoxidase assay for determination of IgG antibodies to measles virus |
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| Authors: | Ludmilla Shani Hava Haikin Israel Sarov |
| Affiliation: | Virology Unit, Faculty of Health Sciences, and Soroka Medical Center, Ben-Gurion University of the Negev, Beer Sheva, Israel |
| Abstract: | A new indirect peroxidase antibody to membrane antigen (IPAMA) technique for the detection of IgG specific antibodies against measles virus is described. The technique utilizes as antigen measles-infected Vero cells dried on glass slides and stored at −70°C. Sera of 50 healthy medical students and laboratory workers and 24 sera of measles, encephalitis, and subacute sclerosing panencephalitis (SSPE) patients were checked by IPAMA and the results have been compared with the results obtained by the hemaglutination-inhibition (HI) test. There is good agreement between the results of both techniques as to the presence or absence of antibody in 48 out of the 50 tested. The advantages of the techniques are discussed. |
| Keywords: | To whom correspondence should be sent. |
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