参芪解毒汤治疗慢性肾功能衰竭的实验研究

目的：验证参芪解毒汤治疗慢性肾功能衰竭（CRF）的疗效。方法：采用腺嘌呤制作大鼠CRF模型，将其分为正常组、模型组、科索亚组及参芪解毒汤高、低剂量组，治疗8周后，测量各组血肌酐(SCr)、尿素氮(BUN)、血清白蛋白（ALB）、甘油三脂(TG)、胆固醇（CHO）、血清瘦素（Leptin）水平，并对大鼠肾脏组织进行病理观察检测转化生长因子β1(TGF-β1)在肾组织的表达。结果：参芪解毒汤高、低剂量组，科索亚组SCr、BUN、Leptin水平均低于模型组，ALB高于模型组（P＜0.05）。病理结果表明，各用药组大鼠的肾组织病理损伤要明显轻于模型组，高剂量组损伤最轻。各用药组TGF-β1在肾组织的表达均明显低于模型组（P＜0.05）。结论：参芪解毒汤可有效降低CRF大鼠SCr、BUN、Leptin水平，升高ALB水平，具有减轻大鼠肾组织的损伤、减少TGF-β1在肾组织的表达、延缓CRF进展的作用。

Effect of the Shenqi Jiedu decoction on chronic renal failure:an experimental study

Objective： To investigate the effects of the Shenqi Jiedu decoction on chronic renal failure(CRF). Methods: Rat models of CRF were established by feeding Adenine. All the rats were randomly divided into five groups: the normal group, the model group, the high dose Shenqi Jiedu decoction group, the low dose Shenqi Jiedu decoction group and the losartan group. After 8 weeks, levels of serum creatinine (SCr), blood urea nitrogen(BUN), plasma albumin(ALB), triglyceride(TG), cholesterol(CHO), Leptin were determined. In addition, the results of pathology were found and the expression of TGF-β1 was determined. Results： Compared with the model group, levels of SCr and BUN in the Shenqi Jiedu decoction groups and the losartan group were decreased while level of ALB was increased (P＜0.05). Pathological results of the Shenqi Jiedu decoction groups and the losartan group were better than those of the model group, and those of the high dose Shenqi Jiedu decoction group were the best. Compared with the model group, expression of TGF-β1 in the Shenqi Jiedu decoction groups and the losartan group were decreased. Conclusions: Shenqi Jiedu decoction can efficiently decrease the SCr, BUN, and Leptin levels and increase the ALB level, reduces the pathological injury and the TGF-β1 expression, and postpones the aggravation of the renal function.