Cyclin E的表达与胆管癌神经浸润及预后关系的研究

目的：检测cyclin E在胆管癌中的表达，并结合多种临床因素探讨其与肿瘤神经浸润及预后的关系。方法：应用S-P免疫组化技术，检测35例胆管癌、25例癌旁正常胆管组织、11例胆总管良性病变组织中cyclin E的表达，并对可能影响预后的因素进行统计学分析。结果：cyclin E在胆管癌组织中表达的阳性率高于癌旁正常胆总管组织（P＜0.01）和胆总管良性病变组织（P＞0.05）；cyclin E表达与肿瘤神经侵犯密切相关（P＜0.01），而与肿瘤大小、分化程度、周围组织浸润及TNM分期无相关性（P＞0.05）；cyclin E阴性组的生存率明显长于阳性组（P＜0.01）。结论：cyclin E在胆管癌中的表达与肿瘤神经侵犯密切相关，cyclin E可作为判断胆管癌预后的指标之一。

Relationship between the expression of cyclin E and nerve infiltration and prognosis in cholangiocarcinoma

Objective: To explore the expression of cyclin E in cholangiocarcinoma and investigate the relevance of cyclin E and several clinical factors with infiltration of nerve and prognosis. Methods: The S-P immunohistochemical staining method was used to determine the expression of cyclin E in 35 cases of cholangiocarcinoma tissues, 25 cases of normal bile duct tissues beside tumor and 11 cases of bile duct′s benign disease. Results: The positive expression rate of cyclin E in cholangiocarcinoma was higher than that in infiltration of surroundings（P＜0.01） and tissues of bile duct′s benign diseases（P＞0.05）. The positive expression of cyclin E was related to infiltration of nerve（P＜0.01）, but was not related to the size of tumor, pathologyic stage, infiltration and clinical TNM stage（P＞0.05）. The median survival time was significantly longer in patients negative for the cyclin E expression than that in those positive for the expression（P＜0.01）. Conclusions: The positive expression of cyclin E is related to infiltration of nerve. cyclin E may be one of markers to evaluate the prognosis of cholangiocarcinoma.