东莞市腹泻患者沙门菌同源性及耐药性特征

目的了解广东省东莞市2007--2009年腹泻患者中沙门菌感染情况和菌株的血清型别、同源性及耐药性变化。方法对纳入研究的腹泻患者进行沙门菌检测，对分离到的菌株进行血清分型、药物敏感试验和脉冲场凝胶电泳（PFGE）分型。结果2007--2009年共检测760份腹泻患者粪便标本，分离到53株沙门菌，检出率为7．0％；共分得15种血清型，鼠伤寒和肠炎沙门菌居多；大多数沙门菌对常用的头孢菌素类和喹诺酮类抗菌药物敏感，除肠炎沙门菌、斯坦利维尔沙门菌和B型副伤寒沙门菌外，其余菌株的血清型无同一PFGE型别的菌株；用XbaI酶切11株肠炎沙门菌可分为PFGE—XbsⅠ 1～6型，其中PFGE-XbaI4型为优势型别。用SfiI和Notl酶对5株肠炎沙门菌进行再分型，综合用XbaI／SfiI／NotI3种酶的结果发现仍有2组菌的PFGE图谱是完全一致的。结论2007--2009年度广东省东莞市沙门菌的感染多数为散发病例，头孢菌素类和喹诺酮类抗菌药物是治疗沙门菌感染的首选药物。

Homology and antibiotic resistant pattern of the Salmonella strains isolated from diarrhea patients in Dongguan City

Objective To understand the Salmonella isolates from diarrhea patients in Dongguan City in terms of serotypes, homology and antibiotic resistance during the period from 2007 to 2009. Methods Salmonella strains were isolated from diarrhea patients and subject to serum agglutination, antibiotic susceptibility testing and pulse-field gel electrophoresis （PFGE） typing. Results A total of 53 （7.0%） Salmonella strains were isolated from 760 stool samples. All these trains were grouped into 15 serotypes. S. typhimurium and S. enteritidis were the most common serotypes. Most o~ the strains were sensitive to cephalosporins and quinolones. All the strains of other serotypes except S. enteritidis, S. stanleyville and S. paratyphi B did not show the same PFGE type. Eleven S. enteritidis strains were classified into six PFGE types following Xba I digestion. The type 4 of PFGE Xba I appeared the most common one. Five S. enteritidis strains were further typed following Sfi I and Not I digestion. Two groups of strains still showed the same PFGE pattern. Conclusions Most Salmonella-associated diarrhea in Dongguan City are sporadic from 2007 to 2009. Cephalosporins and quinolones are the first choice for Salmonella infections.