Secretion of Transforming Growth Factor-ß by Human Myelogenous Leukemic Cells and Its Possible Role in Proliferation of the Leukemic Cells |
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| Authors: | Keiko Komatsu Hiroyuki Nakamura Kiyoko Shinkai Hitoshi Akedo |
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| Affiliation: | Department of Tumor Biochemistry, Research Institute, The Center for Adult Diseases, Osaka, 3-3 Nakamichi 1-chome, Higashinari-ku, Osaka 537 |
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| Abstract: | Transforming growth factor(TGF)-ß activity was found in the neutral extracts of human myelogenous leukemic cells or K562 cells and the conditioned medium from K562 cell culture. BALB/c 3T3 cells grown in soft agar in the presence of TGF-ß1 produced an activity that stimulated the growth of K562 cells. This activity was non-dialyzable. acid-stable, heat-sensitive and partially Inactivated by pronase treatment. These results suggest a mutual growth reliance between the leukemic cells and fibroblasts mediated by paracrine growth factors produced by these cells. |
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| Keywords: | Human myelogenous leukemic cell Fibroblast Transforming growth factor-ß Leukemic cell growth factor |
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