Graves病和桥本病甲状腺细胞凋亡与Fas表达的研究

为了研究正常、Graves病 (GD )和桥本甲状腺炎 (HT )甲状腺细胞凋亡和凋亡相关蛋白Fas的变化特征及其临床意义 ,采用细胞培养方法和流式细胞术检测正常、GD和HT甲状腺细胞凋亡率和Fas表达量。结果发现 :(1)GD和HT甲状腺细胞凋亡率明显高于正常甲状腺细胞 (P <0 0 1)。其中 ,尤以HT甲状腺细胞凋亡增加最为显著 ;(2 )HT甲状腺细胞Fas表达阳性率明显高于正常和GD甲状腺细胞 (P <0 0 1) ,而GD与正常对照相比无统计学差异。以上结果表明 ,自身免疫性甲状腺疾病 (AITD )患者甲状腺细胞存在细胞凋亡和Fas表达的异常变化 ,尤以HT为显著 ,提示Fas介导的细胞凋亡参与AITD的发病过程 ,可能与HT甲状腺细胞破坏有关。

Analysis of Apoptosis and Fas Expression in Thyrocytes from Patients with Autoim mune Thyroid Diseases

To investigate apoptosis and Fas expression in thyrocytes from autoimmune thyroid diseases(AITD),thyroid tissues were obtained from 6 patients with Graves' disease(GD),2 patients with Hashimoto's thyroiditis(HT) and 3 healthy subjects who died accidentally. Thyrocytes were cultured in Eagle'ss medium and apoptosis and Fas expression were detected by flow cytometry. It was found that compared with normal controls,HT thyrocytes showed a significantly higher level of apoptosis associated with a markedly increased expression of Fas,whereas GD thyrocytes only had a relatively higher level of apoptosis. No difference existed in Fas expression between GD and controls. The results suggest that there exists an abnormal apoptosis and Fas expression in thyrocytes from autoimmune thyroid diseases. Fas-mediated apoptosis might be involved in the pathogenesis of AITD,especially in HT.