高效液相色谱法同时测定黄芎抗栓胶囊中5种大黄成分的含量

目的建立高效液相色谱法同时测定黄芎抗栓胶囊中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚含量的方法。方法采用Kromasil C18柱（250mm×4．6mm，5μm），甲醇-0．1％磷酸（85：15）为流动相，流速1．0mL／min，柱温25℃，检测波长254nm。结果芦荟大黄素在0．230～3．68μg（r=0．9998）、大黄酸在0．224～3．584Pg（r=0．9999）、大黄素在0．223～3．568μg（r=0．9998）、大黄酚在0．257～4．112μg（r=0．9999）、大黄素甲醚在0．287～4．592μg（r=0．9998）范围线性良好；平均回收率分别为98．48％、98．10％、99．08％、100．34％、97．52％：RSD分别为1．39％、1．54％、I．44％、2．40％、2．03％。结论该方法简便、可靠、准确，可作为黄芎抗栓胶囊的质量控制方法。

Simultaneous Determination of Five Contents of Rhubarb in Huangxiong Kangshuan Capsule by HPLC

Objective To establish an HPLC method for the determination of Aloeemodin, Rhein, Chrysophanol, Emodin and Physcione in Huangxiong Kangshuan Capsule. Methods The determination was performed on Kromasil-C18 column （250 mm× 4.6 mm, 5 μm） with mobile phase consisted of methanol-0.1% phosphoric acid （85 ： 15） at a flow rate of 1.0 mL/min. Column temperature was 25 ℃. The UV detection wavelength was set at 254 nm. Results The calibration curves were linear in the range of 0.230-3.68μg （r=0.999 8） for Aloeemodin, 0.224-3.584 μg （r=0.999 9） for Rhein, 0.223-3.568 μg （r= 0.999 8） for Emodin, 0.257-4.112 μg （r=0.9999） for Ch .rysophanol and 0.287-4.592 μg （r=0.999 8） for Physcion. The average recovery was 98.48%, 98.10%, 99.08%, 100.34%, 97.52% and RSD was 1.39%, 1.54%, 1.44%, 2.40%, 2.03% respectively. Conclusion The determination method is simple, reliable, accurate, and it can be used for quality control of Huangxiong Kangshuan Capsule.