| 地高辛标记核酸探针技术在家兔视网膜C－sis原癌基因检测中的应用 |
| |
| 引用本文： | 刘旭阳.地高辛标记核酸探针技术在家兔视网膜C－sis原癌基因检测中的应用[J].眼科研究,1994(2). |
| |
| 作者姓名： | 刘旭阳 |
| |
| 作者单位： | 衡阳医学院附二院眼科 |
| |
| 摘 要： | 用ＡＧＰＣ法抽提幼年家兔视网膜总ＲＮＡ，并应用了地高辛标记核酸探针的方法。经斑点杂交显示幼年家兔视网膜出现Ｃ—ｓｉｓ原癌基因表达。对实验方法以及Ｃ－ｓｉｓ原癌基因表达的意义进行了探讨。认为此方法快速、灵敏，检测Ｃ－ｓｉｓ原癌基因表达对深入研究视网膜生长发育及肿瘤等病变有一定意义。
|
| 关 键 词： | 核糖核酸抽提，斑点杂交，视网膜，原癌基因 |
Determination of C-sis Proto-oncogene in Rabbit Retinal Tissue by Technique of Digoxin-labeled
DNA Probe |
| |
| Liu XuyangDepartmen of Ophthalmology,Second Affiliated Hospital,Hengyang Medical College,Hunan.Determination of C-sis Proto-oncogene in Rabbit Retinal Tissue by Technique of Digoxin-labeled
DNA Probe[J].Chinese Ophthalmic Research,1994(2). |
| |
| Authors: | Liu XuyangDepartmen of Ophthalmology Second Affiliated Hospital Hengyang Medical College Hunan |
| |
| Abstract: | otal RNA was extracted by AGPC method.The
technique of digoxin-labeled DNA probe was esta-blished in this experiment.With the dot blot
hybridization,the expression of C-sis proto-oncogene was ob-served in retinal tissue of infant
rabbit The analysis of the experimental procedure suggested that the tech-nique was sensitive
and time saving. It is considered that the determination of C-sis proto-oncogene might playan
important role in the research of growth and disorders. |
| |
| Keywords: | RNA extraction dot blot hybridization retina proto-oncogene |
| 本文献已被 CNKI 等数据库收录！ |
