蓝玉簪颗粒抑制慢性支气管哮喘小鼠气道黏液高分泌及Bcl-2表达

目的 观察蓝玉簪颗粒对慢性支气管哮喘(简称哮喘)小鼠气道上皮杯状细胞化生及黏液高分泌的抑制作用并探讨其机制.方法 32只BABL/C小鼠随机分成正常对照组(A组)、慢性哮喘模型组(B组)、蓝玉簪颗粒组(C组,0.33 g生药/kg体质量)和地塞米松干预组(D组,2.0 mg/kg体质量),每组8只.卵白蛋白腹腔注射致敏并长期雾化吸入制备小鼠慢性哮喘模型.阿尔辛蓝-过碘酸-希夫(AB-PAS)染色观察气道上皮杯状细胞增生、黏液分泌情况,免疫组织化学半定量法测定气道壁Bcl-2含量.计算机图象分析软件测定气道壁Bcl-2、AB-PAS染色阳性面积和气道上皮层面积(Aep)与气道基底膜周径(Pbm)比值(如Aep/Pbm,Bcl-2/Pbm)等.结果 B组小鼠气道上皮层增生,气道黏液分泌显著增加,气道壁Bcl-2表达增强;B组AB-PAS/Pbm,Bcl-2/Pbm等值较C组均显著增高(P＜0.01);B组和C组Aep/Pbm值比较差异有统计学意义(P＜0.01),C组与D组比较差异无统计学意义(P＞0.05);AB-PAS/Pbm与Bcl-2/Pbm呈显著正相关(r=0.671,P＜0.01),Aep/Pbm与Bcl-2表达也呈显著正相关(r=0.784,P＜0.001).结论 蓝玉簪颗粒可以抑制慢性哮喘小鼠气道上皮杯状细胞增殖和黏液高分泌,其部分机制可能通过抑制Bcl-2的表达实现.

Traditional Chinese herb medicine, Lanyuzan granules reduces airway mucus hypersecretion and Bcl-2 expression in murine model of chronic asthma

Objective To evaluate the protective effect of Imnyuzan granules on the airway mucus hypersecretion and Bcl-2 expression in a murine model of chronic bronchial asthma(asthma).Methods Female BABL/C mice(n=32)were randomly divided into four groups:the normal group(group A),the model group (group B),the Lanyuzan granules group(group C.0.33 g/kg body mass)and the dexamethasone group (group D,2.0 mg/kg body mass),the chronic asthmatic model was established with the ovalbumin sensitization and repeated inhalation of ovalbumin aerosols.Lung tissue sections were stained with Alieian Blue-Periodic Acid-Schiff(AB-PAS)for identification of goblet cells and immunostained for assessment of Bcl-2.The area of airway epithelium layer(Aep),the positive staining of AB-PAS and Bcl-2 content in the airway wall were calculated with the computerized image analysis system.Finally,all dimensions were referenced to the perimeter of the basement membrane(Pbm)(e.g.Aep/Pbm).Results The diference of the dimensions,such as the positive staining of AB-PAS/Pbm,the positive staining of Bcl-2/Pbm between group B and group C were significant(P＜0.01):Aep/Pbm in B group was significant higher than that in group C (P＜0.01),but no difference with group D(P＞0.05).A close correlation between the positive staining of AB-PAS and the Bcl-2 content(r=0.671,P＜0.001),and the r=0.784 between the Aep/Pbm and the positive staining of Bcl-2 was significant,too.Conclusions Lanyuzan granules might reduce airways mucus ypersecretion moderately in this model by downregulating Bcl-2 expression.