| 低氧环境下转染血管内皮生长因子165对人胃癌细胞体外侵袭转移的影响 |
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| 引用本文： | 袁翠林,欧希龙.低氧环境下转染血管内皮生长因子165对人胃癌细胞体外侵袭转移的影响[J].东南大学学报(医学版),2011,30(6):896-900. |
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| 作者姓名： | 袁翠林 欧希龙 |
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| 作者单位： | 1. 东南大学医学院,江苏,南京,210009
2. 东南大学医学院,江苏,南京,210009;东南大学附属中大医院,消化科,江苏,南京,210009 |
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| 摘 要： | 目的:探讨低氧环境下血管内皮生长因子165(VEGF-165)对人胃癌细胞侵袭转移的影响及可能的机制。方法:实验分为4组:以利用腺病毒作为载体并已构建成功含VEGF-165基因的重组腺病毒(Ad-VEGF-165)进行瞬时转染,同时用低氧诱导剂氯化钴进行低氧干预的人胃癌细胞株BGC-823为低氧转染(D)组;单纯用低氧诱导剂氯化钴进行低氧干预的人胃癌细胞株BGC-823为单纯低氧干预(B)组;以利用腺病毒作为载体并已构建成功含VEGF-165基因的重组腺病毒(Ad-VEGF-165)进行瞬时转染的人胃癌细胞株BGC-823为单纯转染(C)组;以未转染未行低氧干预的正常细胞作为空白对照(A)组。应用Transwell小室分析低氧环境下VEGF165转染前后胃癌细胞迁移能力的变化;利用半定量RT-PCR方法对4组细胞中尿激酶型纤溶酶原激活物(uPA)、基质金属蛋白质酶2(MMP-2)的mRNA表达水平进行分析。结果:D组胃癌细胞的侵袭能力及uPA、MMP-2的mRNA表达明显高于A、B、C组(P<0.05)。结论:低氧环境和VEGF-165因子共同作用能够明显提高人胃癌细胞BGC-823的侵袭能力及细胞中uPA、MMP-2的mRNA的表达,表明低氧环境下VEGF-165促进肿瘤细胞的侵袭转移。
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| 关 键 词： | 胃癌 血管内皮生长因子165 侵袭能力 尿激酶型纤溶酶原激活物 基质金属蛋白质酶2 氯化钴 |
Effects of VEGF 165 on the invasion and metastasis of human gastric cancer cells in vitro |
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| YUAN Cui-lin,OU Xi-long.Effects of VEGF 165 on the invasion and metastasis of human gastric cancer cells in vitro[J].Journal of Southeast Univ: Medical Sci Ed,2011,30(6):896-900. |
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| Authors: | YUAN Cui-lin OU Xi-long |
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| Institution: | YUAN Cui-lin1,OU Xi-long1,2(1.Clinical Medical College of Southeast University,Nanjing 210009,China,2.Department of Gastroenterology,Zhongda Hospital,Southeast University,China) |
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| Abstract: | Objective: To evaluate the effects of VEGF165 on the invasion and metastasis of human gastric cancer cells line BGC-823 under hypoxia.Methods: The BGC-823 cells were divided into 4 groups,transfected with Ad-VEGF165 transiently and induced by hypoxia mimic cobalt chloride as group D,induced by hypoxia mimic cobalt chloride alone as group B,transfected with Ad-VEGF165 alone as group C,group for non-transfected did not interfere with the normal cell line under hypoxic as blank control(A) group.A transwell cel... |
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| Keywords: | gastric cancer vascular epithelial growth factor 165 transfection urokinase-type plasminogen activator matrix metalloproteinase-2 cobalt chloride |
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