胚胎干细胞移植对急性损伤肝脏的作用

目的 探索胚胎干细胞移植在急性损伤肝脏中的存活和分化情况.方法 用四氯化碳(CC14)制备小鼠急性肝损伤模型,将BrdU标记的来源于小鼠原始生殖细胞的胚胎干细胞经静脉移植于模型动物体内,分别于移植后2周、4周取出肝脏,用免疫组织化学、免疫荧光双重染色和PAS染色方法检测移植细胞在受体小鼠肝内的分布、存活、白蛋白的表达和糖原代谢.结果 急性损伤小鼠肝脏可见大量肝细胞空泡样变性,有严重出血现象;2周时损伤肝内可见炎性细胞浸润和未吸收的出血区;4周时仍见少量炎性细胞浸润.干细胞移植2周,肝小叶内可见散在分布的BrdU和ALB双阳性着色细胞,PAS染色呈阳性反应;部分小细胞呈BrdU单阳性着色;损伤移植组的肝小叶结构基本恢复.移植4周,损伤肝小叶结构正常,小的BrdU阳性细胞数量增多.结论 原始生殖细胞可以在急性损伤肝脏中存活,并分化为肝细胞和肝小叶内的其他细胞,并明显改善损伤肝的组织结构.

THE EFFECT OF TRANSPLANTATION OF MOUSE PRIMORDIAL GERM CELLS ON THE ACUTE DAMAGED LIVER

Objective To investigate the milieu-dependent differentiation of primordial germ cells(PEGs) in the acute damaged liver microenviroment. Methods After PGCs were cultured and proliferated, these cells were labelled with 5-bromo-2-deoxyuridine(BrdU), then transplanted into the acute damaged liver by CCl-4 through tail vein. Two and four weeks later, the liver was extracted and 10μm -cryostat continuous sections were obtained. The existing and differentiation of the transplanted cells were identified by immunohistochemistry, immunofluorescence double staining and histochemistry for BrdU and hepatic-specific ALB, and the glycogen. Results Transplanted PGCs were found to be incorporated into the acute damaged liver and differentiated into hepatocytes, compensating for acute liver failure.Conclusion PGCs can be induced to differentiate into hepatocytes in the acute damaged liver microenvironment, and can be used for cellular hepatoplasty to treat severe liver disease.