Bcl-2和bax蛋白在大鼠脑缺血预处理脑组织中的表达

目的 :探讨凋亡相关基因bcl 2和bax蛋白在脑缺血预处理过程中的表达及意义。方法 :用免疫组织化学SP法分别对假手术对照组、缺血预处理对照组、缺血组和缺血预处理组大鼠前脑组织冰冻切片进行检测。结果 :缺血预处理对照组bcl 2蛋白的表达高于其他 3组 ,而各组bax蛋白表达差异不显著。结论 :缺血预处理对bcl 2蛋白表达的诱导可能是缺血预处理产生抗损伤保护性机制的原因之一

Bcl 2 and baxexpression in rat cerebral ischemic preconditioning

Objective To study the expression of apoptosis regulated genes bcl 2 and bax nocoprotein expression and its significance in rat cerebral ischemic preconditioning. Methods With immunohistochemical assay, bcl 2 and baxexpression were studied in rat brains which were divided into four groups: the sham-operation control group, the cerebral ischemic group and the cerebral ischemic preconditioning group. Results Bcl 2 oncoprotein was induced in cerebral ischemic preconditioning control group. Bax oncoprotein expression had no obviously difference in four groups. Conclusions The expression of bcl 2 and bax oncoprotein after sublethal ischemic preconditioning might be associated with the preconditioning protection against neuronal damage following subsequent lethal ischemia.