日本血吸虫rGST-Sj32蛋白免疫小鼠感染前后细胞因子水平的变化

目的 :为进一步探讨rGST Sj32保护性免疫机制。 方法 :用rGST Sj32加弗氏完全佐剂 (FCA)皮下免疫BALB/c小鼠。于免疫前 ,攻击感染前 (第 3次免疫后 2wk)以及攻击感染后10d、30d及 4 5d ,各剖杀免疫组与对照组小鼠 5只 ,取脾细胞培养 ,对体外培养的脾细胞诱生的细胞因子进行分析。结果 :用rSj32刺激体外培养的小鼠脾细胞时 ,第 3次免疫后2wk(即攻击感染前 )剖杀小鼠的脾细胞分泌IL 4、IL 5和IFN γ的水平与佐剂对照组相比较 ,均有不同程度的升高 ,分别为 ( 10 .2 1± 3.6 5 )ng/L (P >0 .0 5 )、( 19.89± 9.5 7)ng/L (P >0 .0 5 )、( 5 .0 9± 2 .5 1) μg/L (P <0 .0 1)。攻击感染后10d、30d及 4 5d ,对照组与免疫组IFN γ的水平未见升高 ;对照组IL 4和IL 5的水平随着感染时间的延长明显升高 ,免疫组升高不如对照组明显。结论 :rGST Sj32疫苗免疫BALB/c小鼠后 ,可诱导以Th1类细胞因子为主的免疫应答 ;而攻击性感染则诱导以Th2型细胞因子为主的免疫应答

Shistosoma japonicum: dynamics of cytokine production induced by immunization of mice with recombinant GST-Sj32 protein before and after challenge infection

AIM: To explore the mechanism of protective immunity of rGST-Sj32. METHODS: BALB/c mice were immunized subcutaneously with rGST-Sj32 emulsified with Freund's complete adjuvant. Five mice from each group were killed prior to immunization, prior to challenge and 10 days, 30 days and 45 days post-challenge, respectively. Spleen cells from these mice were cultured and levels of cytokine secreted by splenocytes were detected by ELISA. RESULTS: Compared with the control group, the quantity of IL-4, IL-5 and IFN-gamma released from rGST-Sj32-primed splenocytes were increased to varying degrees, being (10.21+/-3.65) ng/L, (19.89+/-9.57) ng/L and (5.09+/-2.51) microg/L, respectively. The level of IFN-gamma post challenge did not increase in control group and immunization group. The levels of IL- 4 and IL-5 in the control group were elevated gradually following challenge, and were much higher than those in immunization group. CONCLUSION: The immunization with rGST-Sj32 elicited mainly a Th1 type immune response in BALB/c mice, whereas the immune response induced by challenge was a Th2 type.