miR-378通过靶向RAB11A促进乳腺癌细胞系的增殖和迁移 |
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引用本文: | 凌晨,卢毓,方珍裕,陆晓磊,张叶飞. miR-378通过靶向RAB11A促进乳腺癌细胞系的增殖和迁移[J]. 浙江中西医结合杂志, 2022, 32(5) |
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作者姓名: | 凌晨 卢毓 方珍裕 陆晓磊 张叶飞 |
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作者单位: | 嘉兴市第一医院,嘉兴市第一医院检验科,嘉兴市第一医院检验科,嘉兴市第一医院检验科,嘉兴市第一医院检验科 |
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基金项目: | 嘉兴市科技计划项目(2021AD30142) |
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摘 要: | 目的 探究miR-378在乳腺癌细胞系中的表达及作用机制。方法 利用TargetScan分析miR-378的作用靶基因RAB11A。利用RT-PCR检测乳腺癌细胞系中miR-378和RAB11A的mRNA水平表达。转染MCF-7和MDA-MB-231细胞miR-378,NC(normal control)对照,采用MTT法和Transwell法检测乳腺癌细胞增殖和迁移能力的变化;采用RT-PCR和Western blot检测RAB11A的表达。荧光素酶报告分析miR-378与靶基因RAB11A之间的信号。结果 miR-378在乳腺癌细胞MCF-7,MDA-MB-231中表达上调;RAB11A在乳腺癌细胞MCF-7,MDA-MB-231中表达下调;在MCF-7,MDA-MB-231细胞中敲降miR-378后,乳腺癌细胞增殖和迁移能力与对照组相比被显著抑制,RAB11A的mRNA和蛋白表达水平明显增加,荧光素酶活性增加。结论 在乳腺癌细胞中,miR-378可通过靶向RAB11A促进乳腺癌细胞的增殖和迁移。
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关 键 词: | miR-378 RAB11A 乳腺癌,增殖,迁移 |
收稿时间: | 2021-07-15 |
修稿时间: | 2022-04-18 |
MiR-378 promotes proliferation and migration of breast cancer by targeting RAB11A |
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Abstract: | Objective To explore the expression and function of miR-378 in breast cancer cells. Methods Using TargetScan to find out how can-miR-378 act on the RAB11A gene, and Rt-PCR verified the expression of miR-378. Transfected MCf-7 and MDA-MB-231 and negative control, CCK-8 and Transwell experiment were applied to verify the proliferation and migration of breast cancer cells. The luciferase reporters were used the miR-378 binding of RAB11A. Results The expression of mir-378 was up-regulated in MCF-7 and MDA-MB-231; Rab11a was down regulated in MCF-7 and MDA-MB-231; the proliferation and migration of breast cancer cells were significantly inhibited after mir-378 was knocked down in MCF-7 and MDA-MB-231 cells, and Rab11a mRNA and protein expression levels were significantly increased, and luciferase activity was increased. Conclusion MiR-378 can promote the proliferation and migration of breast cancer cells by targeting RAB11A. |
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Keywords: | mir-378, Rab11a, breast cancer, proliferation, migration |
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