朝鲜淫羊藿叶的黄酮苷类成分研究

对朝鲜淫羊藿（Epimedium koreanum）叶的化学成分进行研究。利用硅胶柱色谱,Toyopearl,羟丙基葡聚糖凝胶（Sephadex LH-20）柱色谱,ODS反相柱色谱及HPLC等色谱法对朝鲜淫羊藿进行分离和纯化,并通过理化性质和波谱数据分析鉴定其结构。从其干燥叶的70%丙酮/水提取物中共分离得到了6个黄酮苷类化合物,分别鉴定为金圣草素-7-O-β-D-葡萄糖醛酸-6″-甲酯（1）,芹菜素-7-O-β-D-葡萄糖醛酸-6″-甲酯（2）,3’,5,7-三羟基-4’-甲氧基黄酮-3-O-α-L-吡喃鼠李糖基（1→6）-β-D-葡萄糖苷（3）,山奈酚-3-O-α-L-吡喃鼠李糖基（1→6）-β-D-葡萄糖苷（4）,芦丁（5）和槲皮素-3-O-β-D-半乳糖-7-O-β-D-葡萄糖苷（6）。化合物1和2为首次从淫羊藿属植物中分离得到,化合物3,4和6为首次从该植物中分离得到。

Study on the Compositions of Flavonoid Glycosides of Epimedium Koreanum Leaves

Objective： To study the chemical composition of Epimedium koreanum leaves. Methods： The silica gel column chromatography,Toyopearl,Sephadex LH- 20 column chromatography,ODS column chromatography and reversed- phase HPLC chromatography were used to separate and purify Epimedium koreanum and its structures were identified and analyzed through the physical and chemical properties and spectral data. Results： Six flavonoid glycosides were isolated from the dried leaves of 70% of acetone /water extracts,and identified as（1）chrysoeriol- 7- O- β- D- glucuronic acid- 6＂- methyl ester,（2）apigenin- 7- O- β- D- glucuronide- 6＂- methyl ester,（3）3’,5,7- trihydroxy- 4’- methoxy flavone- 3- O- α- L- rhamnopyranosyl（1 →6）- β- D- glucopyranoside,（4）kaempferol- 3- O- α- L- pranrhamnose base（1→6）- β- D- glucopyranoside,（5）rutin and（6） quercetin- 3- O- β- D- galactose- 7- O- β- D- glucopyranoside. Conclusion： The compound 1 and 2 were isolated from Epimedium genus plant for the first time and the compound 3,4 and 6 were isolated from this plant for the first time.