气相色谱法测定丹皮酚软膏中丹皮酚的含量

目的建立用气相色谱法测定丹皮酚软膏中丹皮酚含量的方法。方法采用HP-5极性毛细管色谱柱（（5%苯基）-甲基聚硅氧烷）,氮气为载气,氢焰离子化检测器,甲醇为溶剂,直接进样,外标法计算丹皮酚的含量。结果丹皮酚在5.1-51μg.mL^-1浓度范围内与峰面积线性关系良好（r=0.999 6）,平均加样回收率为99.1%。结论本方法准确度高、精密度好,可以测定丹皮酚软膏中丹皮酚的含量。

Determination of Paeonol in Paeonol Ointment by GC

Objective To establish a method for determination of paeonol in paeonol ointment by GC.Methods The samples were injected into HP-5 Polar capillary columns by direct sampler with hydrogen flame ionized gas detector,carrier gas was nitrogen and methanol was solvent,content of paeonol was calculated in paeonol ointment by external standard method.Results There was excellent linearity between the peak area ratio and concentration of the paeonol in the concentration range of 5.1~51 μg · mL1,the average recovery was 99.1% with RSD of 0.4%(n=9).Conclusions This method was accurate and precise,it can be used for the determination of content of paeonol in paeonol ointment.