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| 单管多重PCR快速检测STD病原菌 | |
| 引用本文： | 唐文志,周玉球,张永良,黄小兰,谭星蓉,黄小燕,李宇雄. 单管多重PCR快速检测STD病原菌[J]. 现代检验医学杂志, 2006, 21(2): 26-28 |
| 作者姓名： | 唐文志 周玉球 张永良 黄小兰 谭星蓉 黄小燕 李宇雄 |
| 作者单位： | 1. 广东省中医院珠海医院,广东,珠海,519015 2. 广东省珠海市妇幼保健院,广东,珠海,519000 3. 广东省珠海市口腔医院,广东,珠海,519000 |
| 基金项目： | 广东省医学科研课题:粤卫(2002)119 |
| 摘 要： | 目的建立一种同时检测解脲支原体(UU)、沙眼衣原体(CT)、淋病奈瑟菌(NG)三种病原体DNA的方法并用于临床标本检测。方法建立单管多重PCR体系,其结果与常规PCR法比较。结果可以一次性检测出解脲支原体、沙眼衣原体和淋病奈瑟菌。结论该法特异性好,单管多重PCR体系检测3种STD病原菌DNA的灵敏度最低为0.43 fg,与单独的PCR检测的灵敏度相同,将它做成体外基因诊断试剂盒能快速特异性指导单种和多重感染。 |
| 关 键 词： | 多重PCR STD病原菌 混合感染 |
| 文章编号： | 1671-7414（2006）02-026-03 |
| 收稿时间： | 2005-10-13 |
| 修稿时间： | 2005-10-13 |
Rapid Detection of Pathogenic Bacteria Using Single-tube Multiplex PCR |
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| TANG Wen-zhi,ZHOU Yu-qiu,ZHANG Yong-liang,HUANG Xiao-lan,TAN Xing-rong,HUANG Xiao-yan,LI Yu-xiong. Rapid Detection of Pathogenic Bacteria Using Single-tube Multiplex PCR[J]. Journal of Modern Laboratory Medicine, 2006, 21(2): 26-28 | |
| Authors: | TANG Wen-zhi ZHOU Yu-qiu ZHANG Yong-liang HUANG Xiao-lan TAN Xing-rong HUANG Xiao-yan LI Yu-xiong |
| Affiliation: | 1. Guangdong Provincial TCM Hospital Zhuhai Hospital ,Guangdong Zhuhai 519015,China ; 2. Guangdong Zhuhai Child and Maternity Health Care Hospital,Guangdong Zhuhai 519000,China; 3. Guangdong Zhuhai Stomatology Hospital, Gua ngdong Zhuhai 519000, China |
| Abstract: | Objective To establish a DNA and apply it in clinical testing approach that can spontaneously detect the three pathogens of Ureplasma urealyticum(UU),Chlamydia trachomatis(CT) and Neisseria gonorrhoeae(NG).Methods Establishing single-tube multiplex PCR system and comparing the results with those of the routine PCR method.Result It could detect UU,CT and NG at one time.Conclusion This method features good specificity,and the lowest sensitivity of the DNA meathod for the measurement of the three kinds of STD pathogenic bacteria is 0.43 fg,and it equals that of the sensitivity of the single-tube multiplex PCR test.Making it into in vitro genetic test kits can swiftly and specifically guide the single and multiple infections. |
| Keywords: | multiplex PCR STD pathogenic bacteria mixed infection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |