| 角蛋白18在HepG2细胞凋亡中的磷酸化(Ser33,Ser52)及其意义 |
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| 引用本文： | 柳雅立,石英,马丽娜,计云霞,魏飞力,吴昊,陈德喜,陈新月.角蛋白18在HepG2细胞凋亡中的磷酸化(Ser33,Ser52)及其意义[J].首都医学院学报,2009,30(6):780-783. |
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| 作者姓名： | 柳雅立 石英 马丽娜 计云霞 魏飞力 吴昊 陈德喜 陈新月 |
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| 作者单位： | 柳雅立,马丽娜,陈新月,LIU Ya-li,MA Li-na,CHEN Xin-yue(首都医科大学附属北京佑安医院国际医疗部);石英,计云霞,魏飞力,吴昊,陈德喜,SHI Ying,JI Yun-xia,WEI Fei-li,WU Hao,CHEN De-xi(首都医科大学附属北京佑安医院性病艾滋病实验室) |
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| 基金项目： | 国家科技重大专项课题，首都医学发展科研基金 |
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| 摘 要： | 目的研究角蛋白18(keratin 18,K18)在人肝癌细胞HepG2凋亡中的磷酸化情况并分析其意义。方法以不同浓度的顺铂(cisplatin,CDDP)作用于HepG2细胞,用双标记流式细胞术(Annexin V/PI)染色检测HepG2细胞的凋亡情况,免疫荧光法和蛋白质印迹法检测不同凋亡状态下K18的磷酸化水平。结果顺铂可以使HepG2细胞发生明显的凋亡,而且凋亡比例随着药物浓度的增加而增加;52位丝氨酸(Ser52)磷酸化水平随着药物浓度增加而增加;33位丝氨酸(Ser33)磷酸化在低浓度药物作用下增加,但在高浓度药物作用下明显减少。结论Ser33和Ser52磷酸化的K18与HepG2细胞凋亡有密切关系。
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| 关 键 词： | 角蛋白18 磷酸化 凋亡 |
| 收稿时间： | 2009-09-22 |
Phosphorylation of Keratin 18(Ser33, Ser52) in Apoptosis of HepG2 Cells and Its Significance |
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| LIU Ya-li,SHI Ying,MA Li-na,JI Yun-xia,WEI Fei-li,WU Hao,CHEN De-xi,CHEN Xin-yue.Phosphorylation of Keratin 18(Ser33, Ser52) in Apoptosis of HepG2 Cells and Its Significance[J].Journal of Capital University of Medical Sciences,2009,30(6):780-783. |
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| Authors: | LIU Ya-li SHI Ying MA Li-na JI Yun-xia WEI Fei-li WU Hao CHEN De-xi CHEN Xin-yue |
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| Institution: | 1. International Medicine Ward, Beijing YouAn Hospital, Capital Medical University;2. STD/AIDS Research Laboratory, Beijing YouAn Hospital, Capital Medical University |
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| Abstract: | Objective To study the phosphorylation of K18 in apoptosis of HepG2 cells and its significance. Methods Apoptosis was induced in HepG2 cells by cisplatin(CDDP). Cells were stained by Annexin V and PI, then analyzed by flow cytometry to evaluate apoptosis. Protein levels of K18 and Ser33, Ser52 phosphorylation of keratin 18 in apoptosis cells were analyzed by immunofluorescence and Western blotting. Results The apoptosis rate of HepG2 cells increased along with the increase of CDDP concentration. The protein level of Ser52 phosphorylated keratin 18 increased along with the concentration of CDDP. The protein level of Ser33 phosphorylated keratin 18 increased with low concentration of CDDP, but decreased evidently with high concentration of CDDP. Conclusion Ser33, Ser52 phosphorylation of keratin 18 has close relationship with apoptosis of HepG2 cells. |
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| Keywords: | keratin 18 phosphorylation apoptosis |
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