Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens |
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| Authors: | Hesselink A T van den Brule A J C Groothuismink Z M A Molano M Berkhof J Meijer C J L M Snijders P J F |
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| Affiliation: | Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands. |
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| Abstract: | We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA. |
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