大鼠“二袖套法”原位肝移植模型的手术体会

目的：建立稳定的大鼠原位肝移植模型。方法：选用健康雄性Wistar大鼠,参考Kamada建立的＂二袖套法＂原位肝移植模型,并加以改良,统计手术各步骤时间以及受体的生存率情况。结果：共进行移植手术86例。供体手术时间为（29.0±0.8）min,受体手术时间为（42.0±0.7）min,无肝期为（15.0±0.3）min。受体术后24h、1周、1个月和3个月的生存率为分别94.2%、91.7%、88.3%、83.7%。结论：改良＂二袖套法＂原位肝移植模型是器官移植基础研究的稳定的动物模型,重视供体的准备是手术成功的关键。

Improvement and experience on two-cuff technique for orthotopic liver transplantation in rats

Objective：To establish a stable and ideal model for orthotopic liver transplantation in rats.Methods：Wistar rats were used as experimental animals.Two-cuff technique was inducted and improved.Operation time of each operative step and survival rate were analyzed respectively.Results：86 cases of transplantation were performed.The operative time of donor was（29.0±0.8） min,the recipient＇s was（42.0±0.7） min and anhepatic phase was（15.0±0.3） min.The survival rate of 24 hours,1 week,1 month and 3 months were 94.2%,91.7%,88.3% and 83.7% respectively.Conclusion：The improvement of two-cuff technique orthotopic transplantation facilitates to establish an ideal and stable animal model for or gan transplantation research.It is a key point for successful transplantation that enough attention is paid on the preparation of graft.