两种检测乙肝表面抗原方法的比较

目的了解两种检测乙型肝炎方法的优缺点,为实验室提供快速、准确的检验方法。方法先后采用胶体金免疫层析测试条法和做血清学检测乙型肝炎表面抗原,并将资料进行整理、分析。结果胶体金免疫层析法检出阳性250份,阳性率为54．82％;ELISA法检出阳性275份,阳性率为60.31％。两法检测结果的总符合率为94．52%。以ELISA法为常规法验证胶体金免疫层析法的敏感性,敏感性为90．91％,特异性为100％。结论胶体金免疫层析法敏感性较酶联免疫法(ELISA)差,认为检测HBsAg仍然以ELISA法为主。

Compare with two detecting methods to HBsAg

Objective To understand that of merits and demerits of two detecting methods to hepatitis B, and to offer a rapid and accurate method for laboratory. Methods Use colloidal gold chromatography test and serologically test defined HBsAg, then collect and analyze the datum. Results 250 positive samples were detected with the colloidal gold chromatography test, with the positive rate 54.82%; 275 positive were detected with the method of ELISA, with positive rate 60.31%. The general coincidence rate between the methods is 94.52%. The sensitivity of colloidal gold chromatography test is 90.91% and the specificity is 100% with the method of ELISA as the routine method to validate. Conclusion The colloidal gold chromatography test is not better than that of ELISA, ELISA should be conducted as the main method to detect HBsAg.