绿茶提取物EGCG对人肝癌细胞株HepG2裸鼠移植瘤超微结构的影响

目的通过复制人肝癌细胞株HepG2裸鼠皮下移植瘤模型，观察绿茶提取物表没食子儿茶素没食子酸酯（EGCG）干预对HepG2移植瘤的作用及其超微结构的影响。方法瘤体接种复制HepG2移植瘤模型，荷瘤裸鼠随机分组，实验组给予EGcG干预3用后处死裸鼠，剥离移植瘤，称重、测量长短径，计算抑瘤率；运用透射电镜观察移植瘤超微结构。结果实验组HepG2移植瘤体积、重量均明显小于对照组（P〈0．05），抑瘤率达31．63％士4．09％；透射电镜观察，实验组HepG2移植瘤组织见不同时期凋亡细胞，部分形成凋亡小体，可见坏死瘤细胞及崩解细胞碎片；对照组呈现细胞形态怪并，核大畸形、电子密度不均，染色质明显，线粒体丰富等恶性肿瘤特有超微结构特点。结论EGCG对人肝癌细胞株HepG2裸鼠皮下移植瘤具有生长抑制作用；对比实验组与对照组移植瘤超微结构特点，推测EGCG可诱导HepG2移植瘤细胞凋亡。

Effect of green tea extractive,EGCG,on ultrastructure of HepG2 transplanted tumor in nude mice

Objective To investigate the effect of green tea extractive,EGCG,on the ultrastructure of human hepatocellular carcinoma cell strain HepG2 transplanted tumor in nude mice.Methods BALB/C(n/n)nude mice were used to replicate HepG2 transplanted tumor model,and randomly divided into experimental and control groups,each nude mouse of experimental group was treated with EGCG.3 weeks After treatment,nude mice were sacrificed,the volume and weight of transplanted tumors were measured,the tumor inhibition ratio(IR) was calculated,and the ultrastructure of transplanted tumors were observed by transmission electron microscope(TEM).Results The volume and weight of HepG2 transplanted tumors in the experimental group were significantly decreased than that in the control group(P<0.05).The tumor inhibition ratio was 31.63%±4.09%.The ultrastructure of HepG2 transplanted tumors were contrasted by TEM,apoptosis and necrosis were showed in the experimental group,apoptosis body was appeared and chromatin was broken into pieces in the stroma;while in the control group,paramorphia of cells,significant nuclear atypia and karyokinesis were showed,lipid droplet and swelling mitochondrion were appeared in the cytoplasm.Conclusion The green tea extractive EGCG may inhibits the growth of HepG2 transplanted tumors in nude mice.EGCG may induce apoptosis of HepG2 transplanted tumor cells.