AG490影响电针足三里干预大鼠T细胞JAK/STAT信号转导途径时效关系研究

目的：探讨氰基苄苯乙烯胺（AG490）影响电针足三里干预大鼠T细胞Janus酪氨酸激酶/信号转导和转录活化因子（JAK/STAT）信号转导途径的时效关系。方法：选用健康成年SpragueDawley雄性大鼠50只,随机分为对照组、足三里穴组、AG490短期组、AG490中期组、AG490长期组。应用流式细胞仪技术,通过免疫荧光染色法测定外周血T淋巴细胞亚群。结果：足三里穴组大鼠CD4＋细胞百分率明显高于对照组（P〈0.01）,CD8＋细胞百分率与对照组相比无统计学意义（P〉0.05）。AG490长期组大鼠CD4＋细胞百分率明显低于足三里穴组（P〈0.01）,AG490中期组大鼠CD4＋细胞百分率低于足三里穴组（P〈0.05）,AG490短期组大鼠CD4＋细胞百分率与足三里穴组相比无统计学意义（P〉0.05）。结论：电针足三里穴可明显提高正常大鼠的T淋巴细胞亚群,AG490可抑制电针足三里穴对T淋巴细胞亚群的调节作用,其抑制效应的强度在一定范围内与AG490的给药时间长度呈正相关。

The time-effect relationship of AG490 on JAK-STAT signaling pathway of T-lymphocyte in rats treated by electroacupuncture at Zusanli

Objective：To observe the time-effect relationship of cyanobenzyl amine styrene（AG490） on JAK-STAT signalling pathway of T-lymphocyte in rats treated by electroacupuncture（EA） at Zusanli. Methods：Fifty Sprague Dawley rats were randomly divided into control group,Zusanli group,short period AG490 group,middle period AG490 group and long period AG490 group. T-lymphocyte subgroups of peripheral blood（including CD4＋ and CD8＋） were measured by direct microvolume whole blood immunoflurescent staining technique with flow cytometer（FCM） evaluate the immune function of rats from different aspects. Results：The value of CD4＋ in Zusanli group was signifincantly higher than that of control group（P0.01）,and the values of CD8＋ between the two groups had no statistical difference（P 0.05）. The value of CD4＋ in long period AG490 group was significantly lower than Zusanli group（P 0.01）,the value of CD4＋ in middle period AG490 group was lower than Zusanli group（P0.05）,the data of the short period AG490 group was not different from that of control group（P 0.05）. Conclusion：EA on Zusanli point can improve cellular immune induced by T-lymphocytes,AG490 can inhibit the regulation effects on T-lymphocytes by EA on Zusanli point. Inhibitory effect was positively correlated with the times of using AG490 in a certain range.