基因芯片技术在乙型肝炎病毒分型和耐药突变检测中的应用 |
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引用本文: | 单万水,徐六妹,吴驰,李兵,韩红星,周伯平. 基因芯片技术在乙型肝炎病毒分型和耐药突变检测中的应用[J]. 国际检验医学杂志, 2008, 29(12): 1069-1071 |
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作者姓名: | 单万水 徐六妹 吴驰 李兵 韩红星 周伯平 |
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作者单位: | 深圳市东湖医院检验科,518020 |
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基金项目: | 深圳市科技计划立项项目 |
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摘 要: | 目的通过基因芯片检测系统,快速检测临床样品中乙型肝炎病毒的基因亚型和耐药突变情况。方法用基因芯片法检测352例临床样品,对慢性乙型肝炎患者感染的HBV亚型及耐药情况进行分析。结果在352例临床病例中,共检出耐药突变病例124例,检出率为35.7%。发现3种基因亚型,其中B型222例,占63.8%;C型115例,占33.0%;D型1例,占0.3%;B、C混合感染9例,占2.6%;型别未分1例,占0.3%。结论PCR与膜芯片杂交技术可检测乙型肝炎病毒基因亚型及耐药突变类型,并具有快速、高通量、敏感的特点,适合各临床医院开展应用。
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关 键 词: | 肝炎病毒 乙型 药物耐受性 突变 基因 寡核苷酸序列分析 |
The application of DNA chip technique in HBV genotyping and detection of drug-resistance mutation |
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Abstract: | Objective To rapidly detect the genopypes of HBV and drug-resistance mutation inclinical samples by means of gene chip detection system.Methods 352 cases of clinical samples were collected.The genopypes of HBV and drug-resistance mutation in the clinical samples were analyzedwith gene chip detection system.Results The detection rate of drug-resistance mutation cases was 35.7%(124/325).Three subtypes were found,in which genotype B was 63.8%(222/352),genotype C was 33.0%(115/352),and genotype D was 0.3%'(1/352).Mixed infection of genotype B and C accounted for 2.6%,and unidentified case was 1(0.3%).Conclusion Combined application of PCR and gene chip technique can be used to detect HBV genotyping and drug-resistance mutation,and it is specific,sensitive and convenient,suitable for application in different levels of hospitals. |
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Keywords: | Hepatitis B virus Drug Tolerance Mutation Genes Oligonucleotide Array Sequence Analysis |
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