| 单纯疱疹病毒Ⅱ型新型DNA疫苗的构建 |
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| 引用本文： | 关蕾,赖维,杨慧兰,朱国兴,许庆芳,陆春. 单纯疱疹病毒Ⅱ型新型DNA疫苗的构建[J]. 岭南皮肤性病科杂志, 2009, 16(1): 15-18 |
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| 作者姓名： | 关蕾 赖维 杨慧兰 朱国兴 许庆芳 陆春 |
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| 作者单位： | 1. 中山大学附属第三医院皮肤性病科,广东,广州,510630
2. 广州军区广州总医院皮肤性病科,广东,广州,510010 |
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| 摘 要： | 目的：针对Ⅱ型单纯疱疹病毒（HSV-2）包膜糖蛋白gD以及包膜糖蛋白gB的CTL表位，构建重组真核表达质粒pVAX—gD—CTL。方法：根据GeneBank提供的HSV-2G株糖蛋白D基因（gD）序列设计PCR引物，以已构建好的pc—gD为模板，特异性扩增HSV-2gm片段，将其克隆于pVAX1载体中构建成质粒pVAX—gD，再将其双酶切后引入CTL片段构建重组真核表达质粒pVAX—gD—CTL。结果：将重组的pVAX—gD—CTL真核表达质粒转化大肠杆菌后，筛选阳性克隆进行酶切及测序鉴定。结论：初步构建HSV-2 pVAX—gD—CTL重组真核表达质粒。
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| 关 键 词： | HSV-2病毒 gD蛋白 CTL表位 DNA疫苗 |
Construction of A New Type Herpes Simplex Virus II DNA Vaccine |
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| GUAN Lei,LAI Wei,YANG Hui-lan,ZHU Guo-xing,XU Qing-fang,LU Chun. Construction of A New Type Herpes Simplex Virus II DNA Vaccine[J]. Southern China Journal of Dermato-Venereology, 2009, 16(1): 15-18 |
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| Authors: | GUAN Lei LAI Wei YANG Hui-lan ZHU Guo-xing XU Qing-fang LU Chun |
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| Affiliation: | 1.Department of Dermatology and Venereology;The Third Affiliated Hospital;Sun Yat-Sen University;Guangzhou 510630;China;2.Department of Dermatology;Liu Hua Qiao Hospital 111 Liu Hua Road;Guangzhou 510010 P.R.China |
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| Abstract: | Objective:To create a new vaccine against HSV,we aimed at constructing a recombinant eukaryotic plasmids pVAX-gD-CTL based on HSV-2 gB and gD protein coding-gene. Methods:HSV-2 gD protein coding-gene was amplified from pc-gD by PCR and inserted to pVAX1 plasmid,together with gB protein coding-CTL epitope.The recombinant plasmid pVAX-gD-CTL was tested in mice by inoculation via intramuscular injection.CTL activity was detected by Lactic Dehydrogenase(LDH) assay.Results:Success of constructing recombinant pla... |
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| Keywords: | HSV-2 gD protein CTL epitope DNA vaccine CTL activity |
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