| 人表皮角质形成细胞的体外改良培养 |
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| 引用本文： | 张娓,冯若,赵飞. 人表皮角质形成细胞的体外改良培养[J]. 郑州大学学报(医学版), 2005, 40(1): 113-115 |
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| 作者姓名： | 张娓 冯若 赵飞 |
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| 作者单位： | 1. 郑州大学基础医学院组织学与胚胎学教研室,郑州,450052
2. 郑州大学第一附属医院皮肤科,郑州,450052 |
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| 摘 要： | 目的:探讨人表皮角质形成细胞的体外改良培养方法。方法:取皮肤标本,先用 2. 5g/L胰蛋白酶 4℃浸泡 22h(冷消化法),以便于表皮真皮分离;分离后刮除真皮面 (真皮刮除法 )以获取基底细胞;收集表皮及基底细胞并混合,制成细胞悬液进行培养。培养至第 5d,采用人工纯化法去除成纤维细胞。其他操作同人表皮细胞常规培养法。培养结束后,以台盼蓝染色法检测细胞存活率,观察细胞生长状态,并与传统培养方法相比较。实验重复 3次。结果:改良组 3次培养细胞存活率分别为 87%, 91%和 95%,均高于对照组 ( 82%, 82%和 85% ) (P<0. 05)。改良组生长速度亦较快。结论:采用人工纯化法、冷消化法和真皮刮除法等改良方法可提高体外培养的人表皮角质细胞存活率及生长速度。
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| 关 键 词： | 细胞培养 角质形成细胞 体外培养 |
| 修稿时间： | 2004-01-06 |
Improved methods of human keratinocyte culturing in vitro |
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| ZHANG Wei ),FENG Ruo ),ZHAO Fei ) ). Improved methods of human keratinocyte culturing in vitro[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(1): 113-115 |
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| Authors: | ZHANG Wei ) FENG Ruo ) ZHAO Fei ) ) |
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| Affiliation: | ZHANG Wei 1),FENG Ruo 1),ZHAO Fei 2) 1)Department of Histology and Embryology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 2) Department of Dermatology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim: To improve the methods of culturing human keratinocytes in vitro.Methods: Manual purification, cool digestion and dermal scratching were used to improve the method of human keratinocyte culturing in vitro.The cell livability was examined by trypan blue staining.The growth speed was detected.The results were compared with those of traditionally cultured group.Results: The cell livability of improved group was higher than that of control group(P<0.05).Cells of improved group growed more rapidly.Conclusion: The improved methods of manual purification, cool digestion and dermal scratching may increase the human keratinocyte livability and growth speed. |
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| Keywords: | cell culture keratinocyte in vitro |
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