西洛他唑对糖尿病大鼠黏附分子的影响

目的　探讨西洛他唑对糖尿病 (DM)大鼠黏附分子CD54、CD10 6、CD62p的表达和坐骨神经传导速度及对肾脏以及主动脉病变的影响。方法　实验设正常组 8只大鼠 ,糖尿病组 8只大鼠 ,胰岛素组 7只大鼠和西洛他唑组 7只大鼠。测定各组坐骨神经传导速度 ,单个核细胞表面CD54、血小板表面CD62p以及肾脏、主动脉CD54或CD10 6的表达水平。结果　西洛他唑明显加快坐骨神经传导速度 (DM组 2 0 3m·s-1± 2 2m·s-1,西洛他唑组 2 8 9m·s-1± 7 9m·s-1,P <0 0 5 )。降低单个核细胞表面CD54的表达 (DM =6 5 1%± 14 9% ,西洛他唑组 2 5 4 %± 8 6 % ,P <0 0 5 ) ,减少肾脏和主动脉CD54和CD10 6表达 ,改善肾脏和主动脉病理变化。而降低血小板表面CD62p的作用不明显 (DM组 4 0 4 %±8 7% ,西洛他唑组 2 8 5 %± 3 7% ,P >0 0 5 )。结论　西洛他唑能降低CD54、CD10 6的表达 ,同时减缓糖尿病大鼠肾和主动脉及坐骨神经病变的发生发展

Effect of cilostazol on adhesion molecules of STZ-induced diabetic rats

OBJECTIVE: To investigate the effect of cilostazol on the expression of CD(54), CD(106), CD(62P) in kidney, aorta, surface of platelet, and mononuclear cell of blood in STZ-induced diabetic rats. METHODS: Rats were randomly divided into four groups: normal control group (n = 8), diabetes control group (n = 8), insulin group (n = 7 subcutaneous injection of insulin, 1 approximately 4 U * d(-1)), and cilostazol group (n = 7, gastric infusion of cilostazol, 18 mg * kg(-1) * d(-1)). Sciatic nerve conductive velocity and level of CD(54)/CD(62p) on the surface of mononuclear cell/platelet were examined by flow cytometry. Immunohistochemistry was used to examine the expression of CD(54) and/or CD(106) in kidney and aorta. RESULTS: The sciatic nerve conductive velocity was 20.3 +/- 2.2 m * s(-1) and 28.9 +/- 7.9 m * s(-1) in diabetic rats and cilostazol group respectively (P < 0.05). The mononuclear cell surface expression of CDa-a(c)54 was 69.1 +/- 14.9% and 25.9 +/- 8.6% in diabetic group and cilostazol group respectively (P < 0.05). The expression of CD(54)/CD(106) in kidney and aorta was decreased. The expression of CD(62p) on the surface of platelets was 40.4 +/- 8.7% in diabetic group and 28.5 +/- 3.7% in cilostazol group (P > 0.05). CONCLUSION: Cilostazol treatment delays the development of diabetic pathological change in kidney, aorta and sciatic nerve and decreases the expression of CD(54) and CD(106).