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| K562细胞经氯化高铁血红素诱导后分化相关基因的筛选 | |
| 引用本文： | 姜立,马文丽,郑文岭,薛社普. K562细胞经氯化高铁血红素诱导后分化相关基因的筛选[J]. 解剖学报, 2001, 32(4): 357-361 |
| 作者姓名： | 姜立 马文丽 郑文岭 薛社普 |
| 作者单位： | 1. 第一军医大学分子生物学研究所 2. 广州军区总医院分子肿瘤研究所 3. 中国医学科学院基础医学研究所 |
| 基金项目： | 国家自然科学基金资助项目 (3 9880 0 3 2 ),广州市重点科技攻关项目 (990 4480 2 2 ) |
| 摘 要： | 目的 研究K562细胞经氯化高铁血红素（Hemin）诱导后在cDNA分子水平上的表达差异。方法 用限制性显示（RD－PCR）方法寻找K562细胞在经Hemin诱导后的cDNA差异表达片段。结果 筛选到差异表达片段4条,并进行了序列测定与同源性分析。结论 K562细胞分化是多基因参与的结果,这些分化相关基因的共同作用使K562细胞向良性方向分化。 |
| 关 键 词： | 限制性显示－PCR 氯化高铁血红素 K562细胞 白血病 Hemin |
SCREENING OF THE DIFFERENTIATION-RELATED GENES FROM K562 CELLS INDUCED BY HEMIN |
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| JIANG Li+,MA Wen li ,ZHENG Wen ling ,XUE She pu. SCREENING OF THE DIFFERENTIATION-RELATED GENES FROM K562 CELLS INDUCED BY HEMIN[J]. Acta Anatomica Sinica, 2001, 32(4): 357-361 | |
| Authors: | JIANG Li+ MA Wen li ZHENG Wen ling XUE She pu |
| Affiliation: | JIANG Li+1,MA Wen li 1*,ZHENG Wen ling 2,XUE She pu 3 |
| Abstract: | Objective In the cDNA level to study the expressing difference of the K562 cells induced by Hemin. Methods Looking for the differential expressing cDNA fragments from the K562 cells induced by Hemin with the method of RD PCR(restriction display PCR). Results Four differential expressing fragments are screened, sequenced and analyzed of autoploidy. Conclusion The differentiation of K562 cells is involved with multiple genes and can be induced to normal under the control of these differentiatial related genes. |
| Keywords: | RD PCR Hemin K562 cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |