Productivity, apoptosis, and infection dynamics of influenza A/PR/8 strains and A/PR/8-based reassortants |
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| Authors: | Isken B Genzel Y Reichl U |
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| Institution: | Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, Sandtorstrasse 1, 39106 Magdeburg, Germany. isken@mpi-magdeburg.mpg.de |
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| Abstract: | In cell culture-based influenza vaccine production significant efforts are directed towards virus seed optimization for maximum yields. Typically, high growth reassortants (HGR) containing backbones of six gene segments of e.g. influenza A/PR/8, are generated from wild type strains. Often, however, HA and TCID50 titres obtained do not meet expectations and further optimization measures are required. |
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| Keywords: | FITC fluorescein isothiocyanate HA hemagglutinin HGR high growth reassortant hpi hours post infection MDCK madin-darby canine kidney MOI multiplicity of infection NA neuraminidase NIBSC National Institute of Biological Standards and Control NP nucleoprotein RKI Robert Koch Institute TCID50 50% tissue culture infective dose |
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