若干分子标志物及临床特征与恶性黑色素瘤预后的多因素相关分析

目的评估若干分子标志物及临床特征对恶性黑色素瘤(MM)预后的影响。方法回顾性分析127例MM患者的临床资料;对127例MM标本以免疫组化法检测HMB45、S-100以及Vimentin蛋白的表达;COX比例风险回归模型对各临床病理指标进行单因素和多因素生存分析。结果 HMB45、S-100以及Vimentin在MM中的阳性表达率分别为89.8%、92.1%和78.0%;单因素分析提示影响MM预后的因素包括年龄、原发病灶是否溃疡、Clark分级、术后病灶切缘情况、AJCC分期、治疗方法及疗效、S-100蛋白(P<0.05);多因素分析发现年龄、术后切缘情况、Clark分级、S-100蛋白以及疗效是影响MM预后的独立因素。结论年龄、术后切缘情况、Clark分级、S-100蛋白与疗效是影响MM预后的独立危险因素,HMB45和Vimentin蛋白表达水平与MM预后不相关。

Multivariate regression analysis of the biomarkers and clinical characteristics in the prognosis of malignant melanoma

Objective To evaluate the impact of the biomarkers and the clinicopathological characteristics on the prognosis of malignant melanoma(MM).Methods The clinical data of 127 MM cases were retrospective analyzed.The surgical specimens of MM were analyzed with immunohistochemistry for detecting HMB45,S-100 and vimentin expressions,and univariate and multivariate regression analysis was performed to analyze their correlation to the prognosis of the patients.Results Among the 127 MM cases,the positivity rates of HMB45,S-100 and vimentin were 89.8%,92.1% and 78.0%,respectively.Univariate analysis showed that the patients’ age,ulcer,Clark classification,postoperative tumor margin,AJCC,treatment outcomes,and S-100 were significantly correlated to the prognosis,and multivariate analysis indicated that age,Clark classification,S-100,tumor margin and outcomes were the independent predictive factors for the prognosis of MM.Conclusion S-100,age,Clark classification,S-100,tumor margin and treatment outcomes were the independent prognostic factors for MM,and HMB45 and vimentin have no predictive value in the prognosis of MM.