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| Transwell和Wound healing在细胞迁移中的应用比较 | |
| 引用本文： | 唐杰,陈龙菊,王军,王配军,陈道帮.Transwell和Wound healing在细胞迁移中的应用比较[J].中国临床解剖学杂志,2007,25(6):687-689. |
| 作者姓名： | 唐杰 陈龙菊 王军 王配军 陈道帮 |
| 作者单位： | 1. 四川大学华西基础与法医学院人体解剖学教研室,成都,610041;郧阳医学院解剖教研室,湖北,十堰,442000 2. 郧阳医学院解剖教研室,湖北,十堰,442000 3. 四川大学华西基础与法医学院人体解剖学教研室,成都,610041 |
| 摘 要： | 目的:比较分析transwell和划痕法(Wound healing)检测细胞迁移能力的差异,探讨两种方法在血清饥饿调节的血管平滑肌细胞迁移中的应用。方法:用DMEM和含10%小牛血清的DMEM分别作用于体外培养的大鼠血管平滑肌细胞24h,采用transwell迁移小室和划痕方法检测血清饥饿(serum starvation)和有血清条件下平滑肌细胞的迁移能力。结果:transwell法和划痕法分别显示了VSMC在血清饥饿组的迁移数目和距离明显比10%血清刺激组少和短。结论:两种方法都能较好地反映细胞的迁移能力,即血清饥饿组明显低于10%血清刺激组。但划痕法更适用于本实验。 |
| 关 键 词： | 划痕 血管平滑肌细胞 血清饥饿 |
| 文章编号： | 1001-165X(2007)06-0687-03 |
| 修稿时间： | 2007年7月10日 |
Comparative study on Transwell and Wound healing methods for detecting vascular smooth muscle cell migration under serum starvation condition |
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| TANG Jie,CHEN Long-ju,WANG Jun,et al..Comparative study on Transwell and Wound healing methods for detecting vascular smooth muscle cell migration under serum starvation condition[J].Chinese Journal of Clinical Anatomy,2007,25(6):687-689. | |
| Authors: | TANG Jie CHEN Long-ju WANG Jun |
| Institution: | TANG Jie,CHEN Long-ju,WANG Jun,et al. Department of Anatomy,West China University of Medical Sciences,Chengdu 610041,China |
| Abstract: | Objective:To investigate the difference between Transwell assay and Wound healing assay for detecting serum starvation-regulated migration of vascular smooth muscle cell (VSMC). Methods:VSMCs were isloated from the male Sprague-Dawley rats adopting the explant method. Effects of serum free culture on the migration of VSMC were detected by Transwell assay and Wound healing assay respectively. Results:The migration ability of VSMC was significantly inhibited by serum starvation compared with 10% FCS/DMEM stimulation. Conclusions:Both of two methods could be used to examine the migration of VSMC under serum starvation condition and Wound healing assay was more suitable to be chosen. |
| Keywords: | Transwell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |