胃肠道P物质、血管活性肠肽在中药大腹皮促动力作用中的变化

目的：探讨大腹皮的促胃肠动力作用机制。方法：32只Wistar大鼠随机分为大腹皮组及对照组，分别给大鼠灌服大腹皮煎液或蒸馏水1、6h后，以葡聚糖蓝－2000为胃肠内标记物观察大鼠胃肠动力变化，免疫组化法观察胃窦及空肠P物质（SP）、血管活性肠肽（VIP）的分布变化。结果：灌服大腹皮煎液1h、6h后，大鼠胃肠动力明显增强（P＜0.01及P＜0.05），胃窦及空肠SP的表达明显增加（P＜0.01及P＜0.05）而VIP的表达明显减少（P＜0.01及P＜0.05），上述变化均以灌服大腹皮煎液1h后为明显，6h后有减弱趋势。结论：大腹皮的促胃肠动力作用可能与胃肠道SP的表达增高及VIP的表达降低有密切关系。

Kinetogenic effects of Pericarpium Arecae on SP and VIP changes in gastrointestinal tract

Objective To study the mechanisms of Pericarpium Arecae. Methods A total of Wistar rats were randomly divided into Pericarpium Arecae group and control group, Pericarpium Arecae decoction or distilled water were given respectively. Changes of gastrointestinal motility rats were assayed by Dextran blue 2000 after 1 and 6 hours. The distributions of SP and VIP in antrum and jejunum were investigated by immunohistochemistry assay. Results The gastrointestinal motility of rats was markedly enhanced ( P <0.01 or P <0.05). The expressions of SP increased significantly ( P <0.01 or P <0.05) and the expressions of VIP decreased significantly ( P <0.01 or P <0.05) in antrum and jejunum of rats at 1 and 6 h after Pericarpium Arecae decoction was given. The changes were more obvious at 1 h than at 6 h. Conclusion The kinetogenic effect of Pericarpium Arecae is closely correlated to the increase of SP expression and the decrease of VIP expression in gastrointestinal tract.