In vitro inhibitory and pro-apoptotic effect of Stellera Chamaejasme LExtract on human lung cancer cell line NCI-H157 |
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| Institution: | Xiaoni Liu,Ning Li,Beijing YouAn Hospital,Capital Medical University,Beijing 100069;China and Institute of Liver Diseases,Beijing 100069,China Yujie Li,Qing Yang,Ying Chen,Xiao-gang Weng,Yiwei Wang,Xiaoxin Zhu,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China |
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| Abstract: | OBJECTIVE:To investigate the inhibitory and pro-apoptotic effect of Stellera Chamaejasme L extract(ESC) in vitro.METHODS:ESC was first extracted with ethanol,and then washed using a polyamide column with 60% ethanol.ESC was then decompressively recycled and vacuum dried at room temperature to obtain active fractions.Subsequently,the cytotoxic and apoptotic effects of ESC on NCI-H157 human lung cancer cells were determined.RESULTS:The results showed that ESC was rich in isomers of Chamaejasminor,neochamaejasmine and Sikokianin.ESC had significant cytotoxicity against NCI-H157 cells,with an IC 50 of approximately 18.50 μg.mL-1.ESC caused significant increase in total apoptotic rate,the activity of caspase 3 and 8,and Fas protein expression(P<0.05).CONCLUSION:The inhibitory effect of ESC on NCI-H157 tumor cells might partly be attributed to its apoptotic induction through activation of the Fas death receptor pathway. |
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| Keywords: | Stellera chamaejasme L Lung neoplasms Apoptosis Antigens CD95 Lissamine rhodamine B |
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