重组新城疫病毒IL29抑制人胃癌细胞系BGC的增殖与迁移

目的探讨表达IL-29的重组新城疫病毒Lo Sota株(rL-IL29)对人胃癌BGC细胞增殖及迁移的影响及其机制。方法将体外培养的人胃癌BGC细胞分为对照组、rL-IL29组和Lo Sota株新城疫病毒(NDV)组,分别经PBS、rL-IL29及NDV感染处理后进行下一步实验。蛋白质印迹检测BGC细胞中IL-29、NDV、P-ERK、MMP2、p-AKT蛋白的表达;CCK8法、划痕试验及Transwell小室法侵袭实验检测胃癌BGC细胞的增殖、迁移能力。结果IL-29蛋白仅在rL-IL29组表达,NDV蛋白在IL-29组及NDV组均稳定表达,且明显高于PBS组(P<0.05);rL-IL29感染BGC细胞后BGC细胞的增殖及迁移能力明显减弱,且rL-IL29的抑制较NDV组作用更强;BGC细胞经rL-IL29感染后P-ERK、MMP2(66 ku/72 ku)、p-AKT蛋白表达较对照组及NDV组明显减弱(P<0.05)。结论rL-IL29抑制胃癌BGC细胞的增殖、侵袭及迁移可能与ERK,AKT等信号通路相关。

rL-IL29 inhibits proliferation and migration of human gastric cancer cell line BGC

Objective To investigate the effect of recombinant Newcastle disease virus expressing IL-29(rL-IL29)on the proliferation and migration with invasion of gastric cancer cells and to explore the potential mechanism.Methods Gastric cancer cell lines BGC were cultured and divided into the PBS,rL-IL29 and newcastle disease virus(NDV)groups infected by the PBS,rL-IL29 or NDV.Western blot was used to detecte the expression of NDV,IL-29,p-ERK,MMP2 and p-AKT protein in cells.The proliferation,migration and invasion of gastric cancer cell lines BGC were detected by CCK8、cell clone forming experiments、scratch test and Transwell invasion test.Results IL-29 protein was only expressed in rL-IL29 group,NDV protein were expressed both in rL-IL29 group and NDV group and was significantly higher than those of PBS group(P<0.05).The proliferation and migration of BGC cells infected with rL-IL29 were inhibited more seriously than those infected with NDV or PBS group.The expression of p-ERK,MMP2(66 ku/72 ku),p-AKT and P65 protein was all significantly decreased(P<0.05).Conclusions The proliferation and migration of gastric cancer cell lines BGC were inhibited by rL-IL29 and the mechanism may be related with ERK,AKT signaling pathways.