牛磺酸联合SN50协同抑制人肝癌细胞系HepG2的增殖

目的探讨牛磺酸联合NF-κB信号通路抑制剂SN50对肝癌细胞系HepG2增殖的影响及其机制。方法将HepG2细胞分为对照组、牛磺酸组(给予150 mmol/L牛磺酸处理24 h)、抑制剂组(给予36μmol/L SN50处理24 h)和牛磺酸+抑制剂组;用MTT法、克隆形成实验和流式细胞仪分别检测细胞的增殖和凋亡;Western blot和RT-qPCR检测细胞中cyclin D1、Bcl-2蛋白和mRNA的表达情况。结果与对照组相比,经150 mmol/L牛磺酸或36μmol/L SN50处理24 h后,HepG2细胞的生存率和集落形成率均明显降低,凋亡率明显升高,细胞中cyclin D1和Bcl-2蛋白和mRNA表达均明显受到抑制(P<0. 05)。同时,SN50增强了牛磺酸对HepG2细胞的增殖和cyclin D1、Bcl-2表达的抑制作用以及对细胞凋亡的促进作用。结论牛磺酸联合NF-κB信号通路抑制剂协同抑制肝癌细胞系HepG2的增殖。

Taurine combined with SN50 inhibits the proliferation of human hepatocellular carcinoma cell line HepG2

Objective To investigate the effect of taurine combined with NF-κB signaling pathway inhibitor SN50 on the proliferation of hepatocellular carcinoma cells line HepG2 and its mechanism. Methods The expression of p-IκBα and IκBα proteins in hepatoma cells line HepG2 was detected by Western blot after the treatment with150 mmol/L taurine for 24 hours. HepG2 cells were randomly divided into control group( untreated),taurine group( treated with 150 mmol/L taurine for 24 hours),inhibitor group( treated with 36 μmol/L SN50 for24 hours) and taurine + inhibitor group( treated with 150 mmol/L taurine and 36 μmol/L SN50 for 24 hours),cell proliferation and apoptosis were examined by MTT,colony forming test and flow cytometry respectively,and cyclin D1,Bcl-2 protein and mRNA were detected by Western blot and RT-qPCR. Results After treatment with150 mmol/L taurine for 24 hours,the expression of p-IκBα protein in HepG2 cells decreased in a time-dependent manner( P<0. 05). After treatment with 150 mmol/L taurine or 36 μmol/l SN50 for 24 hours,the survival rate and cloning rate of HepG2 cells significantly decreased,the apoptosis rate significantly increased,and the expressions of cyclin D1 and Bcl-2 protein and mRNA were significantly inhibited as compared with the control group,all the differences were statistically significant( P<0. 05).At the same time,SN50 enhanced the inhibitory effect of taurine on the proliferation of HepG2 cells,the expression of cyclin D1 and Bcl-2,and the promotion of apoptosis.Conclusions Combined efforts of taurine with NF-κB signaling pathway inhibitor SN50 inhibit strongly the proliferation of hepatoma cells line HepG2 through NF-κB signaling pathway.