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| 七氟醚预处理对局灶性脑缺血再灌注后大鼠空间学习与记忆能力的影响 | |
| 引用本文： | 胡宪文,胡义凤,胡玉萍,刘健,杨建军,李伟彦. 七氟醚预处理对局灶性脑缺血再灌注后大鼠空间学习与记忆能力的影响[J]. 医学研究生学报, 2009, 22(4) |
| 作者姓名： | 胡宪文 胡义凤 胡玉萍 刘健 杨建军 李伟彦 |
| 作者单位： | 1. 安徽医科大学第二附属医院,安徽合肥,230601;南京军区南京总医院麻醉科,江苏南京,210002 2. 徐州医学院江苏省麻醉学重点实验室,江苏徐州,221002 3. 南京军区南京总医院麻醉科,江苏南京,210002 |
| 基金项目： | 南京军区南京总医院科研基金 |
| 摘 要： | 目的:目前,对七氟醚的脑保护功能研究较多,其对认知功能方面的研究相对较少,文中观察七氟醚预处理对局灶性脑缺血再灌注后大鼠空间学习与记忆的影响. 方法:雄性SD大鼠24只,随机分成假手术(C)组,缺血再灌注组(I/R)和七氟醚预处理组(S),每组8只.采用线栓法大脑中动脉阻断前脑血供1h,拔出栓线给予再灌注,七氟醚预处理组术前吸入七氟醚1h,呼气末七氟醚浓度维持在1.0(2.4%)肺泡最低有效浓度(MAC).再灌注72h后利用Morris水迷宫测量术后大鼠空间学习与记忆的能力,用免疫组织化学法测定大鼠海马CA1区胆碱乙酰转移酶(ChAT)的变化. 结果:七氟醚预处理组大鼠逃避潜伏期明显短于缺血再灌注组(P<0.05),在记忆保留实验中七氟醚预处理组大鼠穿越平台次数和平台所在象限探索时间明显多于缺血再灌注组(P<0.05),海马CA1区ChAT阳性细胞的数目七氟醚预处理组也明显多于缺血再灌注组(P<0.05). 结论:1.0 MAC七氟醚预处理1h可能通过保护CA1区胆碱能神经元,对局灶性缺血再灌注大鼠的空间学习与记忆有明显的改善作用. |
| 关 键 词： | 七氟醚 缺血再灌注 学习与记忆 胆碱乙酰化转移酶 |
Sevoflurane preconditioning ameliorates spatial learning and memory after focal cerebral ischemia-reperfusion in rats |
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| HU Xian-wen,HU Yi-feng,HU Yu-ping,LIU Jian,YANG Jian-jun,LI Wei-yan. Sevoflurane preconditioning ameliorates spatial learning and memory after focal cerebral ischemia-reperfusion in rats[J]. Bulletin of Medical Postgraduate, 2009, 22(4) | |
| Authors: | HU Xian-wen HU Yi-feng HU Yu-ping LIU Jian YANG Jian-jun LI Wei-yan |
| Affiliation: | HU Xian-wen1,2,HU Yi-feng3,HU Yu-ping3,LIU Jian1,YANG Jian-jun1,LI Wei-yan1(1.Department of Anesthesiology,Jinling Hospital,Nanjing 210002,Jiangsu,China,2.The Second Hospital of Anhui Medical University,Hefei 230601,Anhui,3.Key Laboratory of Anesthesiology of Jiangsu Province,Xuzhou Medical College,Xuzhou 221002,China) |
| Abstract: | Objective: Sevoflurane has been shown to have the protective effect on the brain,while its effects on learning and memory remain to be further studied.This article aimed to investigate the effect of sevoflurane preconditioning on spatial learning and memory after focal cerebral ischemia-reperfusion in rats.Methods: Twenty-four male SD rats were randomly allocated to 3 groups of equal number: control,ischemia/reperfusion(I/R) and sevoflurane preconditioning(S).The rats were anesthetized with intraperitoneal ... |
| Keywords: | Sevoflurane Ischemia-reperfusion Learning and memory Choline acetyltransferase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |