| 血清酸性α-醋酸萘酯酶的比色测定及初步临床应用 |
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| 引用本文： | 冯春来,黄兴富,范华. 血清酸性α-醋酸萘酯酶的比色测定及初步临床应用[J]. 医学检验与临床, 2008, 19(5) |
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| 作者姓名： | 冯春来 黄兴富 范华 |
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| 作者单位： | 1. 聊城市东昌府区防疫站
2. 东昌府人民医院 |
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| 摘 要： | 目的建立比色测定血清酸性α-醋酸萘酯酶总活性的方法。方法用α-醋酸萘酯为底物,在37℃、pH6.2的酸性环境中经酶水解产生α-萘酚,然后与重氮试剂固蓝B偶联反应显色测定。结果最适pH6.0~6.4,底物浓度为2mmol/L,吸收峰波长为520nm,线性范围0~2500U/L,酶促反应时间为20min,显色反应时间为20min,显色稳定时间为20min,批内、批间变异系数分别为2.67%和5.1%。血红蛋白1g/L和胆红素172umol/L无干扰。枸橼酸钠、草酸盐、肝素、EDTA-K2常用浓度无抑制,氟化钠可抑制该酶活性。标本2~8℃冰箱保存7d结果无变化。正常参考值(n=100)为(1645±378.5)U/L(x-1.96s)。结论该方法简便实用,适于各级实验室开展,可作为评价肝细胞变性坏死、肝纤维化、肝脏合成功能异常的一项新的血清学指标。
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| 关 键 词： | 酸性醋酸萘酯酶 分光光度法 肝病 |
Measurement of acid alpha naphthyl acetate esterase in serum by colorimetric assay and it''s preliminary clinical application |
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| Feng Chunlai,Huang Xingfu,Fan Hua. Measurement of acid alpha naphthyl acetate esterase in serum by colorimetric assay and it''s preliminary clinical application[J]. Medical Laboratory Science and, 2008, 19(5) |
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| Authors: | Feng Chunlai Huang Xingfu Fan Hua |
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| Affiliation: | Feng Chunlai,Huang Xingfu,Fan Hua(Dongchang epidemic prevention station,Liaocheng,252000) |
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| Abstract: | Objective To establish a colorimetric assay for detecting acid alpha naphthyl acetate esterase in serum. Methods When pH was 6.2 and temperature was 37℃ α-naphthyl acetate was, hydrolyzed by acid alpha naphthyl acetate esterase and it produced α-naphthyl. Then colorimetric assay was performed with fast B salt diazotization reaction. Results The optimum pH was 6.0-6.4 and substrate concentration was 2mmol/L. Absorbent wavelength was at 520 nm. Linear range for enzyme determination was from 0 to 2500U/L. Time... |
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| Keywords: | Acid alpha naphthyl acetate esterase Colorimetric analysis Hepatic disease |
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