硫氢化钠后处理对缺氧/复氧心肌细胞线粒体渗透性转换的影响

目的观察硫化氢(hydrogen sulfide,H2S)供体——硫氢化钠(sodium hydrosulfide,NaHS)后处理对培养的新生大鼠心肌细胞缺氧/复氧损伤中线粒体渗透性转换(mitochon-drial permeable transition,MPT)影响。方法将原代培养大鼠乳鼠心肌细胞随机分为缺氧/复氧组(H/R)和硫氢化钠后处理组(H/R+NaHS)。H/R组为缺氧2h后复氧2h。H/R+NaHS组为缺氧2h后,给予1×10-6mol·L-1NaHS后处理0.5h,再复氧1.5h。两组分别于缺氧前、缺氧2h、复氧0.5h、1和2h,应用噻唑蓝法检测细胞活性,荧光指示剂孵育激光共聚焦显微镜下检测线粒体渗透性转换孔(MPTP)的开放程度和线粒体膜电位(Δψm)的变化。结果缺氧2h内细胞活力明显下降,MPTP开放,Δψm下降。在复氧初期两组细胞损伤会进一步加剧,但H/R+NaHS组在NaHS后处理后,细胞活性(45%±3.7%)下降幅度明显小于H/R组(36.5%±1.8%,P<0.05);MPTP荧光强度(52%±5.7%)和Δψm荧光强度(47.2%±5.1%)也明显高于H/R组(40%±5.4%,33.4%±5.0%,P<0.05)。到复氧2h时,两组细胞损伤均不再加重,但H/R+NaHS组各项指标仍然明显好于H/R组(P<0.05)。结论硫氢化钠后处理可以有效调控缺氧/复氧心肌细胞线粒体渗透性转换,阻止MPTP开放,抑制Δψm下降,从而减轻缺氧/复氧造成的心肌细胞损伤。

The effect of NaHS postconditioning on mitochondrial permeable transition during hypoxia/reoxygenation in cultured rat myocardial cells

Aim To explore the effect of NaHS(the donator of H2S) postconditioning on the mitochondrial permeable transition(MPT) in cultured neonatal rat myocardial cells exposed to hypoxia/reoxygenation.Methods Primary cultured myocardial cells from neonatal rats were randomly divided into hypoxia/reoxygenation group(H/R) and NaHS postconditioning group(H/R+NaHS).The cardiomyocytes in H/R group were made hypoxia for 2 hours followed by 2 hours reoxygenation.After 2 hours hypoxia cardiomyocytes in H/R+NaHS group were ...