| 小鼠骨髓源性树突状细胞体外诱导培养体系的构建 |
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| 引用本文： | 刘振明,胡何节,方征东,王晓天,孙小杰,葛新宝.小鼠骨髓源性树突状细胞体外诱导培养体系的构建[J].安徽医科大学学报,2015(8). |
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| 作者姓名： | 刘振明 胡何节 方征东 王晓天 孙小杰 葛新宝 |
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| 作者单位： | 安徽医科大学附属省立医院普通外科,合肥,230001;安徽医科大学附属省立医院普通外科,合肥,230001;安徽医科大学附属省立医院普通外科,合肥,230001;安徽医科大学附属省立医院普通外科,合肥,230001;安徽医科大学附属省立医院普通外科,合肥,230001;安徽医科大学附属省立医院普通外科,合肥,230001 |
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| 摘 要： | 建立一种体外诱导培养小鼠骨髓源性的树突状细胞(DCs)方法,在镜下可观察到培养出的未成熟树突状细胞(imDCs)比成熟树突状细胞(mDCs)细胞突起少。流式细胞术检测 CD80、CD86、MHC-Ⅱ分子在 imDCs 的表达显著低于在 mDCs 上的表达。同种异基因混合淋巴细胞反应显示DCs 刺激 T 细胞增殖的能力随着 DCs 所占比例的增大而增强,在相同比例条件下对刺激 T 细胞增殖的能力,imDCs 显著低于成熟 mDCs。该诱导方法可获得大量符合科研要求的小鼠骨髓源性 DCs。
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| 关 键 词： | 小鼠 树突状细胞 细胞培养 |
A culture system for dendritic cells induced from murine bone marrow in vitro |
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| Abstract: | To establish a culture system for dendritic cells(DCs) induced from murine bone marrow in vitro. Com-pared with mature DCs, fewer spines were observed in immature DCs under microscopy. The expressions of cell surface molecules in immature DCs were significantly lower than those of mature DCs. MLR indicated T cells prolif-eration capacities of same-reaction ratio in immature DCs were significantly lower than those in mature DCs. The methods of culturing DCs establised by us can be used in the future experment. |
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| Keywords: | mice dendritic cells cell culture |
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