骨桥蛋白在 SLE 肾损害 Th17细胞异常中的作用

目的：探讨骨桥蛋白( OPN)在系统性红斑狼疮(SLE)肾损害患者 Th17细胞异常中的作用。方法取40例健康者和50例 SLE 患者(其中，伴肾损害 SLE 患者25例、无肾损害的 SLE 患者25例的血清和肝素钠抗凝血，用ELISA 法检测血清中 OPN 和白介素-17(IL-17)的表达水平，用流式细胞术检测外周血中 CD3+ CD8- IL-17A + T 细胞的阳性率，并分析各组间差异，以及各因素间的相互关系。结果① SLE 肾损害患者组的 OPN、IL-17水平和 CD3+ CD8-IL-17A + T 细胞阳性率显著高于无肾损害的 SLE 患者组、健康对照组，差异均有统计学意义(P <0．01)；无肾损害的 SLE患者组和健康对照组间 OPN、IL-17、CD3+ CD8- IL-17A + T细胞阳性率的差异均无统计学意义(P >0．05)。②血清中OPN 分泌与 IL-17水平、CD3+ CD8- IL-17A + T 细胞阳性率之间均成明显正相关性( P <0．01)。结论外周血中的OPN 对 SLE 肾损害患者的 Th17细胞异常起着重要的调控作用。

The role of osteopontin in abnormal Th17 cell in SLE patients with renal damage

Objective To investigate the regulatory role of osteopontin (OPN) in abnormal Th17 cells of systemic lupus erythematosus (SLE) patients. Methods The levels of OPN and interleukin-17(IL-17) in serum and the rate of CD3 + CD8 - IL-17A + T cell from 40 healthy controls and 50 SLE patients (25 SLE patients with renal dam-age and 25 SLE patients without renal damage) were detected by ELISA and flow cytometry methods. Besides, difference between groups and interaction among multiple elements were analyzed. Results ① The levels of OPN and IL-17 in serum, the rate of CD3 + CD8 - IL-17A + T cell in SLE patients with renal damage all obviously increased than SLE patients without renal damage and healthy controls (P < 0. 01), while there was no difference between SLE patients without renal damage and healthy controls (P > 0. 05). ② Meanwhile, significant positive correlation was shown between serum OPN and serum IL-17 level, CD3 + CD8 - IL-17A + T cell (P < 0. 01). Con-clusion OPN plays an important regulatory role in abnormal Th17 cells of SLE patients with renal damage.