基因芯片检测肝炎肝硬化患者肝组织中HBVDNA

目的 :研究病毒性肝炎基因芯片的制备 ,用肝炎基因诊断芯片 ,免疫组织化学法和原位分子杂交法 ,检测 99例乙型肝炎后肝硬化肝组织中 HBV DNA,比较各种方法优缺点。方法 :将 PCR扩增的 HBVDNA探针用点样仪点于玻片介质上 ,并经过点样处理后制备成基因芯片 ;收集肝炎后肝硬化肝组织标本 99份 ,分别用基因芯片、原位分子杂交法、免疫组织化学法检测 HBVDNA和 HBc Ag。结果 :5 3例 HBc Ag、HBVDNA阳性组织中基因芯片检测阳性 40例 ;HBc Ag阳性 2 2例中基因芯片检测阳性 6例 ;32例 HBc Ag HBVDNA阴性组织中基因芯片检测均阴性。结论 :肝炎基因诊断芯片可以检测肝组织中 HBVDNA,准确率可达 75 % ,假阳性率低。

The expression of HBVDNA in the liver tissues of the patients with posthepatitic cirrhosis by hepatitis virus B with the genechips

Objective: To study the preparation of the gene chips to diagnosed hepatitis virus. It were HBV-DNA and HBcAg in th e liver tissues of the 99 cases patients with posthepatitic cirrhosis by the gen e chips、immunocytochemistry and sita molecule hybridization technique to compar e respective advantages a disadvantages of the icnos methods. Methods: The gene chip was prepared were tested by the HBV-DNA microarrays prepared by spotting h ybridization (PCR) of target genes put on the specialty glass with the lattice a pparatus. The expression of HBV-DNA in the liver tissues of the 99 cases patien ts with posthepatitic cirrhosis by hepatitis virus B were studied with gene chip s immunocytochemistry and sita molecule hybridization technique. Results: In the 53 samples with HBV-DNA and HBcAg positiveb diagnosed with immunolytochemistry and in situ molecule hybridization technique, there were 40 samples tested posi tive by the gene chips. In the 23 samples with HBcAg positive and 32 samples wit h HBcAg and HBV-DNA negative, 6 cases were positive and 32 cases were negative by the gene chip. Conclusion: HBV-DNA in the liver tissues can be detected by the gene chip, the accuracy of diagnosing was 75% and the rate of the sham posit ive was lower. <