Ursolic acid,a naturally occurring triterpenoid,demonstrates anticancer activity on human prostate cancer cells

Purpose Glucocorticoids are widely used as adjuvant therapy in hormonal refractory prostate cancer; their therapeutic role, however, remains unclear. Ursolic acid, a natural triterpene, structurally similar to dexamethasone, exhibits antitumor effects in various cell types. Our main objective was to investigate the effects of ursolic acid on cell viability, apoptosis and bcl-2 protein, in human hormone refractory and androgen-sensitive prostate cancer cells. Methods The ursolic acid-induced changes in cell viability, apoptosis and bcl-2 protein were examined in human hormone refractory prostate cancer PC-3 cells and androgen-sensitive LNCaP cells, by MTT assay, flow cytometry and western blot analysis, respectively. Results Ursolic acid inhibited significantly the cell viability and induced apoptosis in PC-3 cells at 55 μM and in LNCaP cells at 45 μM associated with a downregulation of bcl-2 protein. Conclusions The antiproliferative and apoptotic effects of ursolic acid in PC-3 and LNCaP cells implicate its potential therapeutic use for the treatment of hormone refractory and androgen-sensitive prostate cancer. The downregulation of bcl-2 may be one of the molecular mechanisms via which it induces apoptosis in PC-3 and LNCaP cells. Financial support by a grant PAVE from the General Secretariat of Research and Technology in cooperation with the company ELPEN.