| 糖类及氨基酸层析分析法的改进 |
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| 引用本文： | 王桂红,寇娴,张雁冰,薛华珍,殷明文. 糖类及氨基酸层析分析法的改进[J]. 郑州大学学报(医学版), 2003, 38(6): 966-967 |
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| 作者姓名： | 王桂红 寇娴 张雁冰 薛华珍 殷明文 |
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| 作者单位： | 郑州大学化学系,郑州,450052;郑州大学化学系,郑州,450052;郑州大学化学系,郑州,450052;郑州大学化学系,郑州,450052;郑州大学化学系,郑州,450052 |
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| 摘 要： | 目的：对氨基酸、糖类的层析分析方法进行改进。方法：糖类层析分析时用V(体积分数95％乙醇)：V(浓氨水)=10：0．5(pH=10)作展开剂；氨基酸层析分析时，将显色剂茚三酮直接溶于展开剂中。计算各种糖类及氨基酸的Rf值。结果：各种糖类、氨基酸展层时间、斑点展开距离及显色结果令人满意。结论：改进后的方法简单、快速，结果满意。
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| 关 键 词： | 氨基酸 糖 展开剂 显色剂 层析法 |
| 修稿时间： | 2002-09-22 |
Chromatography analysis improvement in Saccharides and Amino acids |
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| WANG Guihong,KOU Xian,ZHANG Yanbing,XUE Huazhen,YIN Mingwen. Chromatography analysis improvement in Saccharides and Amino acids[J]. Journal of Zhengzhou University: Med Sci, 2003, 38(6): 966-967 |
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| Authors: | WANG Guihong KOU Xian ZHANG Yanbing XUE Huazhen YIN Mingwen |
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| Affiliation: | WANG Guihong,KOU Xian,ZHANG Yanbing,XUE Huazhen,YIN Mingwen Department of Chemistry,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim: To improve chromatography analysis of glucide and amino acids. Method: 95% ethyl alcohol: concentrated ammonia ( V : V =100:5, pH=10) was used as developer of glucide. Developer was combined with spray reagent in amino acid chromatography analysis. The R f for varied glucide and amino acids was calculated. Results and Conclusion: The results were satisfactory and the method is simple and fast. |
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| Keywords: | amino acid glucide developer spray reagent chromatography |
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