慢性肝病患者T淋巴细胞亚群、IL-10和IL-12变化的意义

目的：观察慢性肝病患者T淋巴细胞亚群、IL-10和IL-12与临床表现的关系。 方法：采用双抗体夹心ELISA法与抗体致敏的红细胞花环试验法，对68例慢性乙肝病毒性肝病患者T淋巴细胞亚群、IL-10和IL-12进行平行测定。 结果：不同临床类型慢性肝病患者CD4⁺、CD4⁺/CD8⁺明显低于对照组，而CD8⁺明显高于对照组；慢性肝炎组IL-10、IL-12明显高于对照组；慢性HBeAg阳性肝炎患者CD4⁺、CD4⁺/CD8⁺及IL-12明显低于HBeAg阴性患者，CD8⁺及IL-10明显高于HBeAg阴性患者。 结论：慢性乙肝病毒性肝病患者细胞免疫功能低下，免疫调节紊乱，IL-10、IL-12水平及T淋巴细胞亚群变化与患者HBV携带状态即HBV的复制活跃程度有关。

Significance of changes in T lymphocyte subsets, IL-10 and IL-12 levels in patients with chronic hepatic diseases

Objective To examine the changes of T lymphocyte subsets, IL-10 and IL-12 levels in patients with chronic hepatic diseases. Methods Serum IL-10, IL-12 and T lymphocyte subsets in 68 patients with various clinic types of viral hepatic diseases were detected by double-sandwich ELISA and erythrocyte garland test, respectively. Results CD4 ,CD4 /CD8 in patients with different clinic types of chronic hepatic disease were significantly decreased, and CD8 cells were significantly increased compared with control, and IL-10, IL-12 levels in patients with chronic hepatitis B and hepatocirrhosis markedly increased. CD4 , CD4 /CD8 , and IL-12 in HBeAg positive hepatitis group were markedly lower than those in HBeAg negative hepatitis group. The number of CD8 cells and IL-10 level were significantly higher than those in HBeAg negative hepatitis group. Conclusion The immunological function is disorder in patients with chronic HBV hepatic disease, and CD4 , CD4 / CD8 , IL-10, and IL-12 levels are related to state of HBV replication carrier or the active degree of HBV in patients with chronic hepatic disease.