肝细胞水化状态对细胞某些代谢的影响

目的 探讨不等渗下有肝细胞水化状态改变对某些代谢的影响。方法 动态观察培养鼠肝细胞〖＾３Ｈ〗亮氨酸、〖＾３Ｈ〗ＴｄＲ掺入量，肝细胞膜Ｎａ＾＋／Ｋ＾＋－ＡＴＰ酶及上清液中ＡＬＴ、ＡＳＴ、ＬＤＨ活性。结果 培养１２０ｍｉｎ，与等渗组比较，高渗直细胞（＾３Ｈ）亮氨酸和（＾３Ｈ）ＴｄＲ掺入量分别减少７２．９９％和６５．７０％（Ｐ〈０．００１），低渗组减少１５．９４％和１０．４１％（Ｐ〉０．０５）。高渗组肝

Effects of hepatocellular hydration state on certain kinds of hepatocellular metabolism

Objective To observe certain metabolic changes induced by alterations of hepatocellular hydration state under aniso osmotic condition. Methods Incorporating rate of 3H] leucine into protein and that of 3H]TdR into DNA were measured and the activity of Na +/K + ATPase of hepatocellular membrane and that of ALT, AST and LDH in the supernatant of cell culture observed dynamically. Results After the cells were cultured for 120 min, the incorporating rates of 3H] leucine and 3H]TdR in the hepatocytes of hyper osmotic group were significantly inhibited as compared with the normo osmotic and hypo osmotic groups ( P <0.01 and 0.05). These two rates were closely correlated in all the 3 groups ( P <0.001). The activity of Na +/K + ATPase in the hyper osmotic group was markedly increased but that in the hypo osmotic group decreased as compared with that in the normo osmotic group ( P <0.05 and 0.01). The two incorporating rates in the hypo osmotic group were negatively but those in the hyper osmotic group positively correlated to the activity of Na +/K + ATPase. The activity of ALT, AST and LDH in the supernatant of cell culture was within the normal range. Conclusion The changes in hepatocellular hydration state may be one of the physiological factors to modulate hepatocellular metabolism, in which the activity of Na +/K + ATPase of hepatocellular membrane might regulate the levels of protein and DNA synthesis under this condition.