Human telomerase reverse transcriptase messenger RNA (TERT mRNA) as a tumour marker for early detection of hepatocellular carcinoma |
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| Affiliation: | 1. Department of Internal Medicine, Faculty of Medicine, Cairo University, Giza, Egypt;2. Department of Clinical Pathology, Faculty of Medicine, Cairo University, Giza, Egypt;1. Department of Internal Medicine, Cairo University, Giza, Egypt;2. Department of Chemical Pathology, Cairo University, Giza, Egypt;1. Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas;3. Department of Surgery, University of Texas Southwestern Medical Center, Dallas, Texas;4. Harold C Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas;7. Department of Clinical Sciences, University of Texas Southwestern Medical Center, Dallas, Texas;6. Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan;5. Center for Clinical Management Research, Ann Arbor Veterans Affairs Healthcare Systems, Ann Arbor, Michigan;1. The Department of Clinical & Chemical Pathology, NCI, Cairo University, Egypt;2. The Department of Medical Oncology, NCI, Cairo University, Egypt;1. Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Giza, Egypt;2. Endemic Medicine Department, Faculty of Medicine, Cairo University, Giza, Egypt;3. Oncology Department, Dubai Hospital, Dubai, United Arab Emirates;1. Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Anhui Provincial Hospital, Hefei 230001, PR China;2. Department of Hepatic Surgery, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, PR China;3. Department of Pathology, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, PR China;4. Department of Plastic Surgery, The Second Hospital of Anhui Medical University, Hefei 230601, PR China |
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| Abstract: | Background and study aimsHepatocellular carcinoma (HCC) is the fifth most common malignancy in the world. Although tumour markers such as α-foetoprotein (AFP) are widely used and important for HCC detection in clinical scenes, they still do not provide a satisfactory solution to detect HCC at the early stage. The aim of our study was to illustrate the significance of serum human telomerase reverse transcriptase messenger RNA (hTERT mRNA) as a novel biomarker for early detection of HCC.Patients and methodsThirty-five patients with HCC, 15 patients with liver cirrhosis, and 10 healthy subjects were sex and age matched. History taking, full physical examination, and laboratory investigations including liver function tests, hepatitis markers, AFP, and quantification of serum human telomerase reverse transcriptase mRNA (hTERT mRNA) using real-time reverse transcription polymerase chain reaction (RT-PCR) were conducted. Ultrasonography (US), triphasic computed tomography (CT), and liver biopsy were carried out.ResultsThe hTERT was above the cutoff point (>144 copies/ml) in 27 HCC patients with a sensitivity of 77.14% and a specificity of 100% and with a positive predictive value (PPV) of 100% and a negative predictive value (NPV) of 65.2%. AFP was above the cutoff point (>50 ng/ml) in 23 HCC patients with a sensitivity of 65.71% and a specificity of 96% and with a PPV of 96.3% and an NPV of 53.8%.Our study also showed a statistically significant relationship between the size of the tumour in HCC patients and both AFP and hTERT, and hTERT appears to be more correlated with the size of the tumour than AFP. There is no direct correlation between hTERT or AFP and the number of focal lesions with p value >0.05.ConclusionSerum hTERT mRNA is more sensitive and specific than AFP in the early detection of HCC and its level correlates with the size of the tumour. |
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| Keywords: | Hepatocellular carcinoma Serum human telomerase reverse transcriptase messenger RNA (hTERT mRNA) α-Foetoprotein |
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